Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 101582..102189 Replicon plasmid pBS3d
Accession NZ_CP064935
Organism Rhizobium phaseoli strain BS3

Toxin (Protein)


Gene name hicA Uniprot ID A0A7X6IUV3
Locus tag HER27_RS28005 Protein ID WP_064826346.1
Coordinates 101935..102189 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7X6IWU0
Locus tag HER27_RS28000 Protein ID WP_064826345.1
Coordinates 101582..101938 (-) Length 119 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HER27_RS27980 97928..98074 - 147 Protein_102 calcium-binding protein -
HER27_RS27985 98331..99110 - 780 WP_064826342.1 hypothetical protein -
HER27_RS27990 100384..100839 - 456 WP_064826478.1 hypothetical protein -
HER27_RS27995 101097..101414 + 318 WP_064826344.1 hypothetical protein -
HER27_RS28000 101582..101938 - 357 WP_064826345.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HER27_RS28005 101935..102189 - 255 WP_064826346.1 type II toxin-antitoxin system HicA family toxin Toxin
HER27_RS28010 102403..102978 - 576 WP_037146207.1 dihydrofolate reductase family protein -
HER27_RS28015 103277..104038 - 762 WP_064826347.1 class I SAM-dependent methyltransferase -
HER27_RS28020 104087..104332 - 246 WP_010678589.1 DUF1127 domain-containing protein -
HER27_RS28025 104428..105219 + 792 WP_064826348.1 helix-turn-helix transcriptional regulator -
HER27_RS28030 105141..105512 - 372 WP_064830285.1 hypothetical protein -
HER27_RS28035 105558..106556 - 999 WP_004680351.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gmd 1..1111454 1111454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-73)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9250.61 Da        Isoelectric Point: 10.2030

>T181900 WP_064826346.1 NZ_CP064935:c102189-101935 [Rhizobium phaseoli]
MNSKQRKTLAAVFKDPVSGTIEWAAIENLLVAAGADLIEGNGSRVKFEKDGIIASFHRPHPAKEAKRYQVRDAREFLMQI
GGTP

Download         Length: 255 bp

>T181900 NZ_CP086200:3046587-3046838 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 119 a.a.        Molecular weight: 13271.99 Da        Isoelectric Point: 5.5837

>AT181900 WP_064826345.1 NZ_CP064935:c101938-101582 [Rhizobium phaseoli]
MNIMTYNGYHARIEFDAEDDVFFGRIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLKEAADHFAEARLPA

Download         Length: 357 bp

>AT181900 NZ_CP086200:3046282-3046560 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X6IUV3

Antitoxin

Source ID Structure
AlphaFold DB A0A7X6IWU0

References