Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 84255..84952 Replicon plasmid pBS3b
Accession NZ_CP064933
Organism Rhizobium phaseoli strain BS3

Toxin (Protein)


Gene name vapC Uniprot ID A0A192TIG6
Locus tag HER27_RS24250 Protein ID WP_064826047.1
Coordinates 84255..84689 (-) Length 145 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A246S8D2
Locus tag HER27_RS24255 Protein ID WP_064826046.1
Coordinates 84686..84952 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HER27_RS24230 80204..81556 + 1353 WP_064826050.1 LLM class flavin-dependent oxidoreductase -
HER27_RS24235 81567..82418 + 852 WP_064826049.1 ABC transporter permease -
HER27_RS24240 82457..83440 + 984 WP_064830098.1 ABC transporter substrate-binding protein -
HER27_RS24245 83450..84220 + 771 WP_010020915.1 ABC transporter ATP-binding protein -
HER27_RS24250 84255..84689 - 435 WP_064826047.1 type II toxin-antitoxin system VapC family toxin Toxin
HER27_RS24255 84686..84952 - 267 WP_064826046.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HER27_RS24260 85028..86839 - 1812 WP_064826045.1 chloride channel protein -
HER27_RS24265 87046..87480 + 435 WP_016737028.1 MarR family transcriptional regulator -
HER27_RS24270 87572..88480 - 909 WP_064826044.1 homocysteine S-methyltransferase family protein -
HER27_RS24275 88594..89283 - 690 WP_064826043.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..352110 352110


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-127)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-62)


Sequences

Toxin        


Download         Length: 145 a.a.        Molecular weight: 16116.54 Da        Isoelectric Point: 4.6889

>T181897 WP_064826047.1 NZ_CP064933:c84689-84255 [Rhizobium phaseoli]
VRLLLDTNVLSEVTKPNPDMRVLAWLDKLDEDRTFISIVSIAEIRRGVALMDSGRKRDALAEWLARDLPQRFERRIIPVE
EPVALAWGDLMGQAKRSGRGLSSMDGLIAATAVAHDLTLAIRNTRDFEGFGIELVDPWVDPLDP

Download         Length: 435 bp

>T181897 NZ_CP086200:424094-424228 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9848.06 Da        Isoelectric Point: 6.3615

>AT181897 WP_064826046.1 NZ_CP064933:c84952-84686 [Rhizobium phaseoli]
MQPSRQGDESWTVASAKAKLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRKTARKGTLVEFLMSSPLRGADLDLERQR
DEPRDPEL

Download         Length: 267 bp

>AT181897 NZ_CP086200:423878-424030 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TIG6

Antitoxin

Source ID Structure
AlphaFold DB A0A246S8D2

References