Detailed information of TA system TA181894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1061364..1062037
• Replicon: chromosome
• Accession: NZ_CP064931
• Organism: Rhizobium phaseoli strain BS3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HER27_RS04965
• Protein ID: WP_012484473.1
• Coordinates: 1061624..1062037 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3PTS5
• Locus tag: HER27_RS04960
• Protein ID: WP_012484474.1
• Coordinates: 1061364..1061627 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HER27_RS04945	1056508..1058451	+	1944	WP_004673346.1	PrkA family serine protein kinase	-
HER27_RS04950	1058464..1059738	+	1275	WP_012484476.1	YeaH/YhbH family protein	-
HER27_RS04955	1059735..1061285	+	1551	WP_016733577.1	SpoVR family protein	-
HER27_RS04960	1061364..1061627	+	264	WP_012484474.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HER27_RS04965	1061624..1062037	+	414	WP_012484473.1	type II toxin-antitoxin system VapC family toxin	Toxin
HER27_RS04970	1062160..1063068	+	909	WP_064825466.1	adenylate/guanylate cyclase domain-containing protein	-
HER27_RS04975	1063361..1064569	+	1209	WP_012484471.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (9-57)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15145.58 Da
• Isoelectric Point: 7.2781

>T181894 WP_012484473.1 NZ_CP064931:1061624-1062037 [Rhizobium phaseoli]
MTAWMLDTNIASHVIKGDRPEILRRLAALPMDGIVISSITEGELLYGLAKRDYPKALSERVRQFLVRVDVLPWDHGVTRA
YGDLRAACEAKGTILAPLDMMIAAHAVATAATLVTRDKAFARISEPLKVDDWTSERS

Nucleotide

• Length: 414 bp

>T181894 NZ_CP086198:1283705-1283977 [Macrococcus canis]
ATGCCTAAGAAAAAGATACTATTTTATTCAAGTGGTTTTGAAGATTATACGTACTGGCAAACTAACGATAAAACTGTATT
AAAGAAAATAGATAAACTAATAAAGAGTATTATGATTGATGGTGCAAATAAAGGTATAGGTAAGCCAGAAAAATTATTAG
GAAACTATACAGGTTGCTATAGCAGAAGAATCACTCATGAACATAGGCTCGTATATAAGATAGAAGATGATTCAATTTGT
ATTTTACAATGTCGTTTTCATTATAAAAGGTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10024.18 Da
• Isoelectric Point: 5.1847

>AT181894 WP_012484474.1 NZ_CP064931:1061364-1061627 [Rhizobium phaseoli]
MATKTAKIFTTGRSQAVRLPAEFRFEESEVFVRRDPKTGDVILSRKPDSWDGLFELHRKDPIPDDFLGAADRSQPSQDRD
PFEGWKE

Nucleotide

• Length: 264 bp

>AT181894 NZ_CP086198:1283455-1283712 [Macrococcus canis]
ATGAAAGTCACAACTTATTCTAATGCAAGATCAAATTTTAGAAAAATAATAGATATTGTTAATGAAGACTGTGAACCTTA
CACAATCACAACAAATACGCATAATGCCGTGATATTATCTGAACAAGATTATAATAGTATGATTGAAACACTTTATTTAC
AGAGTACACCAGCGAATGCCGAAAGATTACAAAAAGCGATAGATGAATCAACAGATAAAAATAACTTACTTCAAGTTGAG
ATAGATTTAGATGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M3GIB4