Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 238299..238941 Replicon chromosome
Accession NZ_CP064875
Organism Bacillus toyonensis strain P18

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag I0K03_RS01350 Protein ID WP_000635963.1
Coordinates 238591..238941 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag I0K03_RS01345 Protein ID WP_000004570.1
Coordinates 238299..238586 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I0K03_RS01320 233626..234588 + 963 WP_000961175.1 UV DNA damage repair endonuclease UvsE -
I0K03_RS01325 234581..235153 - 573 WP_000908504.1 rhomboid family intramembrane serine protease -
I0K03_RS01330 235246..235605 + 360 WP_000583422.1 holo-ACP synthase -
I0K03_RS01335 235762..236712 + 951 WP_002059179.1 outer membrane lipoprotein carrier protein LolA -
I0K03_RS01340 236830..237999 + 1170 WP_200203585.1 alanine racemase -
I0K03_RS01345 238299..238586 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
I0K03_RS01350 238591..238941 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
I0K03_RS01355 239010..241178 + 2169 WP_000426215.1 RNA-binding transcriptional accessory protein -
I0K03_RS01360 241237..241353 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
I0K03_RS01365 241561..242019 + 459 WP_070174050.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T181868 WP_000635963.1 NZ_CP064875:238591-238941 [Bacillus toyonensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T181868 NZ_CP086194:309066-309347 [Xanthomonas oryzae]
ATGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCAGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT181868 WP_000004570.1 NZ_CP064875:238299-238586 [Bacillus toyonensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT181868 NZ_CP086194:309365-309655 [Xanthomonas oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References