Detailed information of TA system TA181857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 917988..919615
• Replicon: chromosome
• Accession: NZ_CP064871
• Organism: Xanthomonas oryzae pv. oryzicola strain YN01

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IYN96_RS04240
• Protein ID: WP_229689376.1
• Coordinates: 919055..919615 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: A0A5E8KSB4
• Locus tag: IYN96_RS04235
• Protein ID: WP_047340152.1
• Coordinates: 917988..919058 (-)
• Length: 357 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IYN96_RS04215 (IYN96_04205)	913863..914189	-	327	WP_024710580.1	hypothetical protein	-
IYN96_RS04220 (IYN96_04210)	914493..915773	-	1281	WP_024710581.1	amidohydrolase family protein	-
IYN96_RS04225 (IYN96_04215)	915770..917179	-	1410	WP_047340153.1	amidohydrolase	-
IYN96_RS04230 (IYN96_04220)	917319..917879	+	561	WP_044750878.1	DUF924 family protein	-
IYN96_RS04235 (IYN96_04225)	917988..919058	-	1071	WP_047340152.1	macro domain-containing protein	Antitoxin
IYN96_RS04240 (IYN96_04230)	919055..919615	-	561	WP_229689376.1	DUF4433 domain-containing protein	Toxin
IYN96_RS04245 (IYN96_04235)	920039..920965	-	927	WP_024710586.1	Grx4 family monothiol glutaredoxin	-
IYN96_RS04250 (IYN96_04240)	921011..921802	+	792	WP_024710587.1	polysaccharide deacetylase family protein	-
IYN96_RS04260 (IYN96_04250)	922106..922777	-	672	WP_047340150.1	class I SAM-dependent methyltransferase	-
IYN96_RS04265 (IYN96_04255)	923100..923750	-	651	WP_024710589.1	SGNH/GDSL hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (5-185)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 21558.42 Da
• Isoelectric Point: 7.2479

>T181857 WP_229689376.1 NZ_CP064871:c919615-919055 [Xanthomonas oryzae pv. oryzicola]
MHCGNAAVQSSSWVSIGNPELIDKRSSHPVPLPPGGFSNDYVPFYFTPFLPMLRNIHTAWGGIQRRPNEDIVILVSSLHH
VAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHVPLDGLRGIVCHTDGL
QQTIERQLQERNLKLPVHTRTGWYFR

Nucleotide

• Length: 561 bp

>T181857 NZ_CP086191:c1481003-1480722 [Xanthomonas oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 357 a.a.
• Molecular weight: 39970.97 Da
• Isoelectric Point: 6.0089

>AT181857 WP_047340152.1 NZ_CP064871:c919058-917988 [Xanthomonas oryzae pv. oryzicola]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDRADVRPLIEEVLAGLDTDILVFEPTLKYQNVAKRSG
VEKLTPARALIAELVRRYWVLGMECGLLEIQKLAWFLERNIERAGLPELDLRFSAHKYGPYADRLRHLLNGLDGSYLHCD
KRISDADPLDVIWFDDNRKSFVQTYLKSEAKQYIPALEATAALIDGFESPFGMELLATVDWLIVQEGISAQVADVREGIR
HWPGDQEAAARKDRLFDDRALGIALKRLASGAIAVA

Nucleotide

• Length: 1071 bp

>AT181857 NZ_CP086191:c1480704-1480414 [Xanthomonas oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E8KSB4