Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5127799..5128415 Replicon chromosome
Accession NZ_CP064847
Organism Escherichia coli strain 20

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag IPT86_RS24635 Protein ID WP_001129490.1
Coordinates 5128041..5128415 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag IPT86_RS24630 Protein ID WP_001275523.1
Coordinates 5127799..5128041 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPT86_RS24610 5123034..5123471 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
IPT86_RS24615 5123516..5124457 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
IPT86_RS24620 5124873..5126687 + 1815 WP_000226332.1 hypothetical protein -
IPT86_RS24625 5127361..5127579 + 219 WP_001251290.1 ribbon-helix-helix domain-containing protein -
IPT86_RS24630 5127799..5128041 + 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
IPT86_RS24635 5128041..5128415 + 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
IPT86_RS24640 5128452..5129381 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
IPT86_RS24645 5129378..5130013 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
IPT86_RS24650 5130010..5130912 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T181837 WP_001129490.1 NZ_CP064847:5128041-5128415 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T181837 NZ_CP086183:4124065-4124394 [Xanthomonas oryzae]
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT181837 WP_001275523.1 NZ_CP064847:5127799-5128041 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT181837 NZ_CP086183:4124391-4124810 [Xanthomonas oryzae]
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AAGTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCGTTTTCTGATCGAGAACAA
AGTCAAATCCATTGCCATCCCGCCACTGGGCGCGGGCAATGGAGGATTGGACTGGGCCGAGGTGCGCCCACTGATCGAAG
AAGTACTAGGGAACCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References