Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 4735150..4735762 Replicon chromosome
Accession NZ_CP064847
Organism Escherichia coli strain 20

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag IPT86_RS22840 Protein ID WP_000833473.1
Coordinates 4735577..4735762 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1M2IVK0
Locus tag IPT86_RS22835 Protein ID WP_000499742.1
Coordinates 4735150..4735560 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPT86_RS22820 4730607..4731422 - 816 WP_000891820.1 AraC family transcriptional regulator -
IPT86_RS22825 4731648..4733048 + 1401 WP_000204814.1 MFS transporter -
IPT86_RS22830 4733059..4735038 + 1980 WP_001026863.1 glycoside hydrolase family 127 protein -
IPT86_RS22835 4735150..4735560 - 411 WP_000499742.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IPT86_RS22840 4735577..4735762 - 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
IPT86_RS22845 4736235..4737314 + 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
IPT86_RS22850 4737355..4738893 - 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
IPT86_RS22855 4739001..4740296 - 1296 WP_000985736.1 Fic family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T181836 WP_000833473.1 NZ_CP064847:c4735762-4735577 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T181836 NZ_CP086183:2334530-2334781 [Xanthomonas oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15242.13 Da        Isoelectric Point: 4.4482

>AT181836 WP_000499742.1 NZ_CP064847:c4735560-4735150 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT181836 NZ_CP086183:2334225-2334503 [Xanthomonas oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCTAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IVK0

References