Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3915028..3915682 Replicon chromosome
Accession NZ_CP064847
Organism Escherichia coli strain 20

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag IPT86_RS18865 Protein ID WP_000244781.1
Coordinates 3915028..3915435 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IPT86_RS18870 Protein ID WP_000354046.1
Coordinates 3915416..3915682 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPT86_RS18845 3910985..3912718 - 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -
IPT86_RS18850 3912724..3913434 - 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IPT86_RS18855 3913459..3914355 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
IPT86_RS18860 3914467..3914988 + 522 WP_001055874.1 flavodoxin FldB -
IPT86_RS18865 3915028..3915435 - 408 WP_000244781.1 protein YgfX Toxin
IPT86_RS18870 3915416..3915682 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IPT86_RS18875 3915925..3916905 + 981 WP_000886072.1 tRNA-modifying protein YgfZ -
IPT86_RS18880 3916982..3917641 - 660 WP_000250274.1 hemolysin III family protein -
IPT86_RS18885 3917805..3918116 - 312 WP_001182965.1 N(4)-acetylcytidine aminohydrolase -
IPT86_RS18890 3918161..3919594 + 1434 WP_137490915.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T181832 WP_000244781.1 NZ_CP064847:c3915435-3915028 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T181832 NZ_CP086182:c4240500-4240348 [Xanthomonas oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT181832 WP_000354046.1 NZ_CP064847:c3915682-3915416 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT181832 NZ_CP086182:c4240284-4240150 [Xanthomonas oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References