Detailed information of TA system TA181831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2865420..2866204
• Replicon: chromosome
• Accession: NZ_CP064847
• Organism: Escherichia coli strain 20

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: IPT86_RS14040
• Protein ID: WP_000613626.1
• Coordinates: 2865710..2866204 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: IPT86_RS14035
• Protein ID: WP_000718446.1
• Coordinates: 2865420..2865713 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPT86_RS14025	2860581..2861540	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
IPT86_RS14030	2862113..2865286	+	3174	WP_106106802.1	ribonuclease E	-
IPT86_RS14035	2865420..2865713	+	294	WP_000718446.1	DUF1778 domain-containing protein	Antitoxin
IPT86_RS14040	2865710..2866204	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
IPT86_RS14045	2866299..2867252	-	954	WP_001212748.1	flagellar hook-associated protein FlgL	-
IPT86_RS14050	2867264..2868907	-	1644	WP_000096449.1	flagellar hook-associated protein FlgK	-
IPT86_RS14055	2868973..2869914	-	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
IPT86_RS14060	2869914..2871011	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T181831 WP_000613626.1 NZ_CP064847:2865710-2866204 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T181831 NZ_CP086182:c4240284-4240150 [Xanthomonas oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11053.63 Da
• Isoelectric Point: 10.5423

>AT181831 WP_000718446.1 NZ_CP064847:2865420-2865713 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT181831 NZ_CP086182:c4240500-4240348 [Xanthomonas oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Structures

Toxin

• AlphaFold DB: V0T0H9