Detailed information of TA system TA181821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1524422..1525127
• Replicon: chromosome
• Accession: NZ_CP064847
• Organism: Escherichia coli strain 20

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: IPT86_RS07415
• Protein ID: WP_000539524.1
• Coordinates: 1524741..1525127 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IPT86_RS07410
• Protein ID: WP_001280945.1
• Coordinates: 1524422..1524751 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPT86_RS07395	1519580..1520491	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
IPT86_RS07400	1520668..1523016	+	2349	WP_000950331.1	EAL domain-containing protein	-
IPT86_RS07405	1523024..1524352	+	1329	WP_106106846.1	GGDEF domain-containing protein	-
IPT86_RS07410	1524422..1524751	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
IPT86_RS07415	1524741..1525127	-	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IPT86_RS07420	1525353..1526678	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
IPT86_RS07425	1526891..1527274	+	384	WP_000497137.1	biofilm formation regulator BssR	-
IPT86_RS07430	1527385..1528500	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
IPT86_RS07435	1528497..1529123	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T181821 WP_000539524.1 NZ_CP064847:c1525127-1524741 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T181821 NZ_CP086180:4233087-4233338 [Xanthomonas oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT181821 WP_001280945.1 NZ_CP064847:c1524751-1524422 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT181821 NZ_CP086180:4232782-4233060 [Xanthomonas oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCTAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7