Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1145344..1145962 Replicon chromosome
Accession NZ_CP064847
Organism Escherichia coli strain 20

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IPT86_RS05615 Protein ID WP_001291435.1
Coordinates 1145344..1145562 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IPT86_RS05620 Protein ID WP_000344800.1
Coordinates 1145588..1145962 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPT86_RS05580 1140633..1141205 + 573 WP_000779831.1 YbaY family lipoprotein -
IPT86_RS05585 1141236..1141547 - 312 WP_000409911.1 MGMT family protein -
IPT86_RS05595 1141926..1142279 + 354 WP_000878140.1 DUF1428 domain-containing protein -
IPT86_RS05600 1142321..1143871 - 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IPT86_RS05605 1144035..1144505 - 471 WP_000136192.1 YlaC family protein -
IPT86_RS05610 1144621..1145172 - 552 WP_000102574.1 maltose O-acetyltransferase -
IPT86_RS05615 1145344..1145562 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IPT86_RS05620 1145588..1145962 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IPT86_RS05625 1146508..1149657 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IPT86_RS05630 1149680..1150873 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T181819 WP_001291435.1 NZ_CP064847:c1145562-1145344 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T181819 NZ_CP086180:2377587-2377868 [Xanthomonas oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT181819 WP_000344800.1 NZ_CP064847:c1145962-1145588 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT181819 NZ_CP086180:2377886-2378176 [Xanthomonas oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References