181818

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1111035..1111872 Replicon chromosome
Accession NZ_CP064847
Organism Escherichia coli strain 20

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag IPT86_RS05445 Protein ID WP_000227782.1
Coordinates 1111035..1111577 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag IPT86_RS05450 Protein ID WP_001353405.1
Coordinates 1111561..1111872 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPT86_RS05420 1106113..1106703 - 591 WP_001276309.1 protein deglycase YajL -
IPT86_RS05425 1106666..1107577 - 912 WP_000705836.1 2-dehydropantoate 2-reductase -
IPT86_RS05430 1107745..1108236 + 492 WP_001138904.1 nucleotide binding protein YajQ -
IPT86_RS05435 1108364..1109728 - 1365 WP_001000978.1 MFS transporter -
IPT86_RS05440 1110135..1110979 + 845 Protein_1059 sel1 repeat family protein -
IPT86_RS05445 1111035..1111577 - 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
IPT86_RS05450 1111561..1111872 - 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
IPT86_RS05455 1112057..1112947 - 891 WP_000971327.1 heme o synthase -
IPT86_RS05460 1112959..1113288 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
IPT86_RS05465 1113288..1113902 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
IPT86_RS05470 1113892..1115883 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
IPT86_RS05475 1115905..1116852 - 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T181818 WP_000227782.1 NZ_CP064847:c1111577-1111035 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T181818 NZ_CP086180:c1764401-1764249 [Xanthomonas oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT181818 WP_001353405.1 NZ_CP064847:c1111872-1111561 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT181818 NZ_CP086180:c1764185-1764051 [Xanthomonas oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References