Detailed information of TA system TA181815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 586872..587448
• Replicon: chromosome
• Accession: NZ_CP064847
• Organism: Escherichia coli strain 20

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A2U2VE01
• Locus tag: IPT86_RS02850
• Protein ID: WP_001131965.1
• Coordinates: 586872..587159 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: IPT86_RS02855
• Protein ID: WP_106106778.1
• Coordinates: 587146..587448 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPT86_RS02820	582281..583693	-	1413	WP_000199309.1	PLP-dependent aminotransferase family protein	-
IPT86_RS02825	583870..584034	+	165	WP_000394263.1	DUF1127 domain-containing protein	-
IPT86_RS02830	584076..584414	-	339	WP_000132633.1	endoribonuclease SymE	-
IPT86_RS02835	584629..585297	-	669	Protein_549	DUF3387 domain-containing protein	-
IPT86_RS02840	585291..585464	-	174	Protein_550	SAM-dependent DNA methyltransferase	-
IPT86_RS02845	585509..586675	-	1167	WP_000800823.1	restriction endonuclease	-
IPT86_RS02850	586872..587159	+	288	WP_001131965.1	BrnT family toxin	Toxin
IPT86_RS02855	587146..587448	+	303	WP_106106778.1	BrnA antitoxin family protein	Antitoxin
IPT86_RS02860	587482..588438	-	957	WP_001361361.1	GTPase	-
IPT86_RS02865	588449..588652	-	204	WP_000467859.1	YbdD/YjiX family protein	-
IPT86_RS02870	588702..590852	-	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-
IPT86_RS02875	591120..592340	-	1221	WP_000873756.1	amino acid deaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11223.71 Da
• Isoelectric Point: 7.4021

>T181815 WP_001131965.1 NZ_CP064847:586872-587159 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T181815 NZ_CP086179:4033784-4034194 [Streptomyces fungicidicus]
GTGATCTACCTGCTCGACACGTCCGGCCTGGTCCGGCTGCTCCGCGATCCGAAACTGCAATCGGCCTGGTACGACGCGAT
CGATGCCGGGGCCATCGCATCCTGCTACGTGCAACGCGCCGAGTTCCTCCACAGTGCCCGGAACGGGCGCGAGTACGACG
AGATCACGGAAATGTTCACCGACCTCTACCCCGACGTGTCGGTACCGAAGAACGCGGGGCGCTGGATCGGCGCGGTGCAG
CACCGCATGGCTCGGGCAGGGGAGCATCGCAGCGCCTCGGCGGTGGACCTCCTCATCGCCGCCACCGCGGCCCACCATGG
CCTGGCTGTCCTCCACGATGATGCCGACTATCGGGCGATTGCCCGGCACGCATCCGACCTGACCGAGCACAACGTCCACG
ACATCGCTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11550.04 Da
• Isoelectric Point: 9.4646

>AT181815 WP_106106778.1 NZ_CP064847:587146-587448 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQIRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT181815 NZ_CP086179:4033566-4033787 [Streptomyces fungicidicus]
ATGTCCGTGACTCAGATTGACATCGACGACGACGCCCTGGAGCGAGCCATGGCTCTGGCCCACGTCCGGACCAAGAAGGA
GGCGGTCAACCTCGCTCTGAATTTCTATGCCGAGCAGCAGGAGCGTGCGGCGCGCATCAGCCGTCACTTCGAGCGTGCCC
GTGAGTGGGGTGCCGTTGAGGACGCCGAGCGTCTGCACAGGGCGGAGAAGCGCAACCGGTGA

Structures

Toxin

• AlphaFold DB: A0A2U2VE01