Detailed information of TA system TA181813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 480603..481198
• Replicon: chromosome
• Accession: NZ_CP064847
• Organism: Escherichia coli strain 20

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: IPT86_RS02375
• Protein ID: WP_000239581.1
• Coordinates: 480848..481198 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: IPT86_RS02370
• Protein ID: WP_001223213.1
• Coordinates: 480603..480854 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPT86_RS02360	476268..480047	+	3780	WP_075861634.1	autotransporter assembly complex protein TamB	-
IPT86_RS02365	480050..480391	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
IPT86_RS02370	480603..480854	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
IPT86_RS02375	480848..481198	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
IPT86_RS02380	481277..481807	-	531	WP_000055075.1	inorganic diphosphatase	-
IPT86_RS02385	482117..483073	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
IPT86_RS02390	483206..484708	+	1503	WP_001095665.1	sugar ABC transporter ATP-binding protein	-
IPT86_RS02395	484722..485744	+	1023	WP_001383778.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T181813 WP_000239581.1 NZ_CP064847:480848-481198 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T181813 NZ_CP086179:c2966079-2965711 [Streptomyces fungicidicus]
ATGTACTACCTCACCTTGCCCGAGTTGCTGAACCTCGCGAAGCGGCTCGGGGAGGACGTGGTGCGCGACTACGGGCTGCT
CGACTCGGCCTTGGCGCGTCCGCAGTCGAGCGTGTTCGGCCAGGACGCCTACCCGGACGTGTGGCAGAAGGCCGCGGCAC
TGATGGAGTCCCTCGCTCGCAACCATGCCCTCGTCGACGGGAACAAGCGCCTCGCCTGGTACGCGACCTGGGTCTTCCTG
CATATGAACGGGCATCCGCTCGACCCGGACTTCGACGTGGACGAGGCGGAGCAGTTCGTGCTGGACGTCTGCCAGGGCGC
ACTGGACGTCCCCAAGATCGCAGCTCAGTTGCCCCGCTTCGCGCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT181813 WP_001223213.1 NZ_CP064847:480603-480854 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT181813 NZ_CP086179:c2966276-2966079 [Streptomyces fungicidicus]
ATGGCTGGACTGAACCTGCGGTTTACTGACGAAGAGCTCGATGCCCTGCGTGAGCGTGCGGCTGCGGAAGGGCGCAGCAT
GCAGACCTTCGCGCACGACGCGGTGATCAAGGCCATAAACGAACACTCGAGGCTGTTCAACGAGGCGGCCGACCACGTGC
TGAGGGCCAGCGAAGAGCTGAACCGGAGGCTCGCCTGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7