Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 302353..302850 Replicon chromosome
Accession NZ_CP064847
Organism Escherichia coli strain 20

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IPT86_RS01445 Protein ID WP_001173343.1
Coordinates 302677..302850 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IPT86_RS01440 Protein ID WP_000398619.1
Coordinates 302353..302649 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPT86_RS01420 299082..299801 - 720 WP_000611288.1 two-component system response regulator DcuR -
IPT86_RS01425 299798..301429 - 1632 WP_001216471.1 sensor histidine kinase -
IPT86_RS01430 301610..301840 + 231 WP_000371713.1 (4Fe-4S)-binding protein -
IPT86_RS01435 301852..302124 + 273 WP_000405647.1 N-acetyltransferase -
IPT86_RS01440 302353..302649 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IPT86_RS01445 302677..302850 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IPT86_RS01450 302969..304486 - 1518 WP_000003800.1 lysine--tRNA ligase -
IPT86_RS01455 304723..306180 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T181811 WP_001173343.1 NZ_CP064847:302677-302850 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T181811 NZ_CP086178:3241776-3242042 [Streptomyces fungicidicus]
GTGAGTAGCGGCTACCGCACCGTCTTCCGGCCGGAGGCTCAGGCGGAGCTTCGCAAGATTCCCCGGGACATGGCCCTGAG
GATCCTGGTCAAACTGACGGAACTGGAAACGGACCCGCTCGGCTTCCGCACCACGGCGCTCGTCTCCCGGCCCGACCGGC
GCCGGCTCCGTGTCGGCGACTACCGCGTCATCTACACGATCGACAACGGCGAGTTGACGGTCTGGGTCGTTCATGTCGGA
CACCGCTCCACGGTATACGACACCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT181811 WP_000398619.1 NZ_CP064847:302353-302649 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT181811 NZ_CP086178:3241517-3241783 [Streptomyces fungicidicus]
ATGACCGAGATGCCCATAGAGTCCATTCGTGACGTGCGCGCCCACCTGGCCGAGGTGGTCGAACGGGCGGACCGGGACGA
CACGCCCACGGTCATCACCCGGCGAGGCAAGCAGGTGGCCGCAGTCGTGTCGATGGAGGTCCTTCGCAAGTACCAGGAAT
GGGAAGAGCGCGAGATCAACCGGATCATCGACGAGCGCATGGCGAACCCGGCAGCCGGTATCCCGATCGAGGACGTCATG
CGGGAGACGCTGGAGCGCGGTGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References