Detailed information of TA system TA181766

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1139952..1140750
• Replicon: chromosome
• Accession: NZ_CP064825
• Organism: Escherichia coli UTI89

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: IIE09_RS05405
• Protein ID: WP_001094434.1
• Coordinates: 1140376..1140750 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IIE09_RS05400
• Protein ID: WP_001552747.1
• Coordinates: 1139952..1140329 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IIE09_RS05370	1136830..1137651	+	822	WP_001234585.1	DUF945 domain-containing protein	-
IIE09_RS05375	1137651..1137896	+	246	WP_001164966.1	hypothetical protein	-
IIE09_RS05380	1137990..1138463	+	474	WP_001542276.1	antirestriction protein	-
IIE09_RS05385	1138479..1138955	+	477	WP_001186727.1	RadC family protein	-
IIE09_RS05390	1139018..1139239	+	222	WP_000692315.1	DUF987 domain-containing protein	-
IIE09_RS05395	1139258..1139902	+	645	WP_000086759.1	hypothetical protein	-
IIE09_RS05400	1139952..1140329	+	378	WP_001552747.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
IIE09_RS05405	1140376..1140750	+	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
IIE09_RS05410	1140747..1141238	+	492	WP_000976859.1	hypothetical protein	-
IIE09_RS05415	1141250..1141447	+	198	WP_000839257.1	DUF957 domain-containing protein	-
IIE09_RS05420	1141540..1142382	+	843	WP_001280460.1	DUF4942 domain-containing protein	-
IIE09_RS05430	1142853..1143791	+	939	WP_000351298.1	glyoxylate/hydroxypyruvate reductase GhrA	-
IIE09_RS05435	1143846..1144583	+	738	WP_000283664.1	zinc-binding phosphatase	-
IIE09_RS05440	1144607..1145161	+	555	WP_001001909.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T181766 WP_001094434.1 NZ_CP064825:1140376-1140750 [Escherichia coli UTI89]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T181766 NZ_CP086128:c4436043-4435510 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13757.63 Da
• Isoelectric Point: 6.3127

>AT181766 WP_001552747.1 NZ_CP064825:1139952-1140329 [Escherichia coli UTI89]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT181766 NZ_CP086128:c4436320-4436054 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: Q1RDD2