Detailed information of TA system TA181741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1604479..1605115
• Replicon: chromosome
• Accession: NZ_CP064818
• Organism: Bacillus subtilis strain SH1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: ITP52_RS07970
• Protein ID: WP_003156187.1
• Coordinates: 1604479..1604829 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: ITP52_RS07975
• Protein ID: WP_003225183.1
• Coordinates: 1604834..1605115 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITP52_RS07930	1599523..1600122	-	600	WP_003246608.1	phosphoserine phosphatase RsbX	-
ITP52_RS07935	1600122..1600910	-	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
ITP52_RS07940	1600876..1601358	-	483	WP_003234299.1	anti-sigma B factor RsbW	-
ITP52_RS07945	1601355..1601684	-	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
ITP52_RS07950	1601746..1602753	-	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
ITP52_RS07955	1602765..1603166	-	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
ITP52_RS07960	1603170..1603535	-	366	WP_003225190.1	RsbT antagonist protein RsbS	-
ITP52_RS07965	1603540..1604364	-	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
ITP52_RS07970	1604479..1604829	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
ITP52_RS07975	1604834..1605115	-	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
ITP52_RS07980	1605231..1606400	-	1170	WP_015252766.1	alanine racemase	-
ITP52_RS07985	1606514..1607530	-	1017	WP_072557167.1	outer membrane lipoprotein carrier protein LolA	-
ITP52_RS07990	1607696..1608061	-	366	WP_015252768.1	holo-ACP synthase	-
ITP52_RS07995	1608156..1608755	+	600	WP_041850724.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T181741 WP_003156187.1 NZ_CP064818:c1604829-1604479 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T181741 NZ_CP086118:2454133-2454417 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT181741 WP_003225183.1 NZ_CP064818:c1605115-1604834 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT181741 NZ_CP086118:2453895-2454143 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32