Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 800605..801521 Replicon chromosome
Accession NZ_CP064818
Organism Bacillus subtilis strain SH1

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag ITP52_RS03705 Protein ID WP_003244695.1
Coordinates 800605..801351 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag ITP52_RS03710 Protein ID WP_003232646.1
Coordinates 801351..801521 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITP52_RS03680 795687..795833 - 147 WP_120363335.1 hypothetical protein -
ITP52_RS03685 795934..796884 - 951 WP_041344800.1 ring-cleaving dioxygenase -
ITP52_RS03690 797272..798588 + 1317 WP_041850924.1 serine/threonine exchanger -
ITP52_RS03695 798864..799481 + 618 WP_003218470.1 DUF47 domain-containing protein -
ITP52_RS03700 799494..800495 + 1002 WP_003232642.1 inorganic phosphate transporter -
ITP52_RS03705 800605..801351 + 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
ITP52_RS03710 801351..801521 + 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
ITP52_RS03715 801607..801744 + 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
ITP52_RS03720 801781..802674 - 894 WP_041850925.1 N-acetylmuramoyl-L-alanine amidase -
ITP52_RS03725 802687..802950 - 264 WP_003232653.1 phage holin -
ITP52_RS03730 802963..803232 - 270 WP_003232655.1 hemolysin XhlA family protein -
ITP52_RS03735 803285..804124 - 840 WP_041850926.1 phage-like element PBSX protein XepA -
ITP52_RS03740 804168..804332 - 165 WP_041850927.1 XkdX family protein -
ITP52_RS03745 804329..804658 - 330 WP_041850928.1 XkdW family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T181740 WP_003244695.1 NZ_CP064818:800605-801351 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T181740 NZ_CP086118:2112809-2112912 [Salmonella enterica subsp. enterica serovar Typhimurium]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT181740 WP_003232646.1 NZ_CP064818:801351-801521 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT181740 NZ_CP086118:c2112914-2112769 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References