Detailed information of TA system TA181734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4811065..4811701
• Replicon: chromosome
• Accession: NZ_CP064793
• Organism: Priestia megaterium strain IGA-FME-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: IMX31_RS24885
• Protein ID: WP_013055004.1
• Coordinates: 4811065..4811415 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: IMX31_RS24890
• Protein ID: WP_013055003.1
• Coordinates: 4811420..4811701 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMX31_RS24850	4806647..4807441	-	795	WP_013055011.1	RNA polymerase sigma factor SigB	-
IMX31_RS24855	4807407..4807892	-	486	WP_025753516.1	anti-sigma B factor RsbW	-
IMX31_RS24860	4807889..4808221	-	333	WP_013055009.1	anti-sigma factor antagonist	-
IMX31_RS24865	4808281..4809291	-	1011	WP_195781426.1	PP2C family protein-serine/threonine phosphatase	-
IMX31_RS24870	4809305..4809706	-	402	WP_013055007.1	anti-sigma regulatory factor	-
IMX31_RS24875	4809710..4810066	-	357	WP_013055006.1	STAS domain-containing protein	-
IMX31_RS24880	4810069..4810905	-	837	WP_013055005.1	STAS domain-containing protein	-
IMX31_RS24885	4811065..4811415	-	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
IMX31_RS24890	4811420..4811701	-	282	WP_013055003.1	hypothetical protein	Antitoxin
IMX31_RS24895	4811902..4813092	-	1191	WP_195781427.1	alanine racemase	-
IMX31_RS24900	4813206..4814264	-	1059	WP_195781428.1	outer membrane lipoprotein carrier protein LolA	-
IMX31_RS24905	4814323..4814688	-	366	WP_195783335.1	holo-ACP synthase	-
IMX31_RS24910	4814753..4815355	+	603	WP_098250182.1	rhomboid family intramembrane serine protease	-
IMX31_RS24915	4815364..4816335	-	972	WP_195781429.1	UV DNA damage repair endonuclease UvsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T181734 WP_013055004.1 NZ_CP064793:c4811415-4811065 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T181734 NZ_CP086118:206805-207290 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT181734 WP_013055003.1 NZ_CP064793:c4811701-4811420 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT181734 NZ_CP086118:206530-206817 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9