Detailed information of TA system TA181731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3792384..3792974
• Replicon: chromosome
• Accession: NZ_CP064784
• Organism: Klebsiella sp. BDA134-6

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: IYV58_RS17520
• Protein ID: WP_071583017.1
• Coordinates: 3792384..3792716 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: IYV58_RS17525
• Protein ID: WP_195729162.1
• Coordinates: 3792717..3792974 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IYV58_RS17510	3788785..3790025	+	1241	Protein_3419	integrase arm-type DNA-binding domain-containing protein	-
IYV58_RS17515	3790647..3791531	-	885	WP_195729161.1	integrase domain-containing protein	-
IYV58_RS17520	3792384..3792716	-	333	WP_071583017.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IYV58_RS17525	3792717..3792974	-	258	WP_195729162.1	antitoxin	Antitoxin
IYV58_RS17530	3793322..3793528	-	207	WP_171996115.1	helix-turn-helix domain-containing protein	-
IYV58_RS17535	3793525..3793998	-	474	WP_195729163.1	hypothetical protein	-
IYV58_RS17540	3794276..3795286	-	1011	WP_195729164.1	DUF4917 family protein	-
IYV58_RS17545	3795573..3796808	+	1236	WP_195729165.1	DNA cytosine methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11772.52 Da
• Isoelectric Point: 9.2926

>T181731 WP_071583017.1 NZ_CP064784:c3792716-3792384 [Klebsiella sp. BDA134-6]
MDRGEIWLVSLDPTSGHEQSGKRPVLIVSKASFNKLTQLPVIVPVTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T181731 NZ_CP086112:579820-580224 [Skermanella rosea]
ATGCTGCGCTTCATGCTCGACACCAATGTTTGCATCCGCGTCCTTCGCGATCGGCCCCCGAGCGCAAGGATCCGGTTCAA
CGCGGAATCGGCTAGCCTCTGCATTTCCACGGTGACGCTGGCCGAATTGCTGCACGGTGCCGCGAAATCCGGACGTCCCA
TCGAGAAACGACATGAGGTGGAGCGCTTGGTTGCCCGCCTGGAGGTACTGCCCTTCGATGACGAGGCGGCGGCTCATTAC
GGCGAGATCCGCGCCGACCTCGAACGTAGCGGCACCATCATCGGTCCCTATGACCTGATGATCGCGGGTCACGCCCGAAG
CCGCGGATTGGTCGTCGTTACCGGCAACCTTGGTGAATTCTCAAGGGTGGACGGGCTTCGCTGCGAAGACTGGCTTGAAG
CCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9296.75 Da
• Isoelectric Point: 4.5821

>AT181731 WP_195729162.1 NZ_CP064784:c3792974-3792717 [Klebsiella sp. BDA134-6]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLVIEPQKRPRYSLEELLAQCDPHAEISEESREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT181731 NZ_CP086112:579596-579820 [Skermanella rosea]
ATGGCTATCCGCACGAGCCTCTTCCTCTCCAATCGGAGCCAAGCCGTAAGGCTTCCAAAAGATGTTGCGTTTCCGCCGAA
TGTCCGGGAAGTCGTTATTCTCCGTGAAGGGTCCCGGCGGGTGATCGTGCCGGCAGACAGTCTTTGGGACGATTTCTTCA
ACTCTCCCGGTGTCGATTTTCCGGACCGGGATCAGCCCGCCACGCAAGAGCGTGAAGCTTTCTGA