Detailed information of TA system TA181719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2176372..2176786
• Replicon: chromosome
• Accession: NZ_CP064781
• Organism: Azospira restricta strain DSM 18626

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: IWH25_RS10705
• Protein ID: WP_203385794.1
• Coordinates: 2176372..2176503 (+)
• Length: 44 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: IWH25_RS10710
• Protein ID: WP_203385795.1
• Coordinates: 2176520..2176786 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IWH25_RS10685	2172531..2173919	+	1389	WP_203385790.1	V-type ATP synthase subunit B	-
IWH25_RS10690	2173916..2174521	+	606	WP_203385791.1	hypothetical protein	-
IWH25_RS10695	2174892..2175392	+	501	WP_203385792.1	HNH endonuclease	-
IWH25_RS10700	2175764..2176210	+	447	WP_203385793.1	hypothetical protein	-
IWH25_RS10705	2176372..2176503	+	132	WP_203385794.1	BrnT family toxin	Toxin
IWH25_RS10710	2176520..2176786	+	267	WP_203385795.1	BrnA antitoxin family protein	Antitoxin
IWH25_RS10715	2177101..2177316	+	216	WP_203385796.1	hypothetical protein	-
IWH25_RS10720	2177536..2178237	+	702	WP_203385797.1	hypothetical protein	-
IWH25_RS10725	2178234..2179391	+	1158	WP_203385798.1	M48 family metalloprotease	-
IWH25_RS10730	2179388..2179960	+	573	WP_203385799.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (19-81)

Sequences

Toxin

Protein

• Length: 44 a.a.
• Molecular weight: 5121.09 Da
• Isoelectric Point: 11.4912

>T181719 WP_203385794.1 NZ_CP064781:2176372-2176503 [Azospira restricta]
MIALAPKTGILYYVAFVDRGKVRRIISLRRANRREVKHYVENF

Nucleotide

• Length: 132 bp

>T181719 NZ_CP086111:c1342359-1342060 [Skermanella rosea]
GTGGCGGTGCTTGAGTGGCGGGAAACGGCCCGCGCCGATCTCCTGGCAATACTTGATTACATATCAGATGACAATTTGGA
CGCCGCTCAGCGTCTAAAGAACGACATTGAGGCCAAGACTGCAAAGCTGCCTGAACATCCGAAGCTCTACCGGACCGGCC
GAGTCGCCGGCACACGCGAAATGGTGGTTCGAACAAATTACGTAGTTGTCTATACCGAAGACACTCGCGCGGTGACGATC
CTTCGCGTGCTTCACGCCGCCCAACAATGGCCGCCTGTGCAGGAGTGGAAAAGCGTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9856.41 Da
• Isoelectric Point: 10.6893

>AT181719 WP_203385795.1 NZ_CP064781:2176520-2176786 [Azospira restricta]
MPTVEEDRAITAAARSDPDAQPLTPKQLKTMVPLKALRGRPKSENKKLLVSVRYSPEVIAYFKSTGEGWQSLMDSVLRKY
VARHSRSA

Nucleotide

• Length: 267 bp

>AT181719 NZ_CP086111:c1342645-1342346 [Skermanella rosea]
ATGGTTGCCCAGACATCAATGCTGCACATCCGCGTGGATGACAAACTCAAGGCTGACGCAACGGAAAAGCTCGCCAATGT
CGGCCTCACTGTCTCGGATGCCGTACGTATCTTGCTTACGCGCATTGCCAAAGAAGGCGCGCTGCCGATCGGATTGACTG
CGACACCTGAAGCTTATGACGAATGGTTTCGGGCCAAGGTTCACGAGGCGCTGGCCGACACAAGCCCAGCGGCACCTCAT
CAGCATGTCATGGACGAAGCTCAAGCCTTGATCGACAGGAAACGTCGTGGCGGTGCTTGA