Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6953..7596 Replicon plasmid pHS20R14-KPC-2
Accession NZ_CP064771
Organism Klebsiella pneumoniae subsp. pneumoniae strain HS20R14 isolate HS20R14

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HS20R14_RS27265 Protein ID WP_001044770.1
Coordinates 7180..7596 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HS20R14_RS27260 Protein ID WP_001261282.1
Coordinates 6953..7183 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS20R14_RS27220 2166..2468 + 303 WP_004197636.1 hypothetical protein -
HS20R14_RS27225 (HS20R14_27170) 2916..3710 - 795 WP_004197635.1 site-specific integrase -
HS20R14_RS27230 (HS20R14_27175) 3908..4924 - 1017 WP_017899884.1 hypothetical protein -
HS20R14_RS27235 (HS20R14_27180) 4935..5249 - 315 WP_053389906.1 hypothetical protein -
HS20R14_RS27240 (HS20R14_27185) 5276..5671 - 396 WP_017899885.1 hypothetical protein -
HS20R14_RS27245 (HS20R14_27190) 5840..6145 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
HS20R14_RS27250 (HS20R14_27195) 6147..6365 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
HS20R14_RS27255 (HS20R14_27200) 6535..6996 - 462 WP_160880186.1 hypothetical protein -
HS20R14_RS27260 (HS20R14_27205) 6953..7183 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HS20R14_RS27265 (HS20R14_27210) 7180..7596 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HS20R14_RS27270 (HS20R14_27215) 7670..9232 + 1563 WP_004206609.1 AAA family ATPase -
HS20R14_RS27275 (HS20R14_27220) 9217..10239 + 1023 WP_000361404.1 helicase UvrD -
HS20R14_RS27280 (HS20R14_27225) 10495..11192 + 698 WP_223174630.1 IS1 family transposase -
HS20R14_RS27285 (HS20R14_27230) 11221..11493 + 273 Protein_15 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..108776 108776


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T181694 WP_001044770.1 NZ_CP064771:7180-7596 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T181694 NZ_CP086100:c2832073-2831970 [Enterobacter ludwigii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT181694 WP_001261282.1 NZ_CP064771:6953-7183 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT181694 NZ_CP086100:2831958-2832108 [Enterobacter ludwigii]
TTTATAATAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCT
AAAAGTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References