Detailed information of TA system TA181693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5840..6365
• Replicon: plasmid pHS20R14-KPC-2
• Accession: NZ_CP064771
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain HS20R14 isolate HS20R14

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: HS20R14_RS27245
• Protein ID: WP_013023785.1
• Coordinates: 5840..6145 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: HS20R14_RS27250
• Protein ID: WP_001568025.1
• Coordinates: 6147..6365 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS20R14_RS27215 (HS20R14_27160)	1543..2169	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
HS20R14_RS27220	2166..2468	+	303	WP_004197636.1	hypothetical protein	-
HS20R14_RS27225 (HS20R14_27170)	2916..3710	-	795	WP_004197635.1	site-specific integrase	-
HS20R14_RS27230 (HS20R14_27175)	3908..4924	-	1017	WP_017899884.1	hypothetical protein	-
HS20R14_RS27235 (HS20R14_27180)	4935..5249	-	315	WP_053389906.1	hypothetical protein	-
HS20R14_RS27240 (HS20R14_27185)	5276..5671	-	396	WP_017899885.1	hypothetical protein	-
HS20R14_RS27245 (HS20R14_27190)	5840..6145	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
HS20R14_RS27250 (HS20R14_27195)	6147..6365	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HS20R14_RS27255 (HS20R14_27200)	6535..6996	-	462	WP_160880186.1	hypothetical protein	-
HS20R14_RS27260 (HS20R14_27205)	6953..7183	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	-
HS20R14_RS27265 (HS20R14_27210)	7180..7596	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	-
HS20R14_RS27270 (HS20R14_27215)	7670..9232	+	1563	WP_004206609.1	AAA family ATPase	-
HS20R14_RS27275 (HS20R14_27220)	9217..10239	+	1023	WP_000361404.1	helicase UvrD	-
HS20R14_RS27280 (HS20R14_27225)	10495..11192	+	698	WP_223174630.1	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..108776	108776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T181693 WP_013023785.1 NZ_CP064771:c6145-5840 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T181693 NZ_CP086100:c1036728-1036510 [Enterobacter ludwigii]
ATGTCTGAAAAACCACTCACAAAAATCGATTATTTGATGCGTTTGCGACGCTGTCAGTCAATAGACACGCTTGAACGTGT
AATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCGGCTGACCACCGTCTTGCAGAAC
TGACTATGAATAAGCTGTATGACAAAATCCCAACGTCCGTGTGGAGATTTGTCCGTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT181693 WP_001568025.1 NZ_CP064771:c6365-6147 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT181693 NZ_CP086100:c1037130-1036756 [Enterobacter ludwigii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTAAACGAGTTGATCGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGATGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAATGCGCAAGATTTGCAGAAATGGCGTAAATCGGGCAACCGACT
ATTTCGTTGCTTCATCAACGTGAGCAGAGCTAACCCCGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8