Detailed information of TA system TA181692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 163188..163831
• Replicon: plasmid pHS20R14-1
• Accession: NZ_CP064769
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain HS20R14 isolate HS20R14

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HS20R14_RS26850
• Protein ID: WP_014386165.1
• Coordinates: 163415..163831 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HS20R14_RS26845
• Protein ID: WP_032408893.1
• Coordinates: 163188..163418 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS20R14_RS26820 (HS20R14_26765)	158261..159244	-	984	WP_001471744.1	NAD(P)H-quinone oxidoreductase	-
HS20R14_RS26825 (HS20R14_26770)	159263..160411	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
HS20R14_RS26830 (HS20R14_26775)	160582..161739	-	1158	WP_032408895.1	acetylornithine deacetylase	-
HS20R14_RS26835 (HS20R14_26780)	161755..162429	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
HS20R14_RS26840 (HS20R14_26785)	162434..162868	-	435	WP_000103648.1	RidA family protein	-
HS20R14_RS26845 (HS20R14_26790)	163188..163418	+	231	WP_032408893.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HS20R14_RS26850 (HS20R14_26795)	163415..163831	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
HS20R14_RS26855 (HS20R14_26800)	164154..165122	+	969	WP_074422984.1	IS5-like element IS903B family transposase	-
HS20R14_RS26860 (HS20R14_26805)	165302..165676	-	375	WP_032408891.1	hypothetical protein	-
HS20R14_RS26865 (HS20R14_26810)	165732..166058	-	327	WP_004152639.1	hypothetical protein	-
HS20R14_RS26870 (HS20R14_26815)	166055..166783	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
HS20R14_RS26875 (HS20R14_26820)	166780..167211	-	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A)	-	1..184047	184047
	-	flank	IS/Tn	-	-	164154..165122	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T181692 WP_014386165.1 NZ_CP064769:163415-163831 [Klebsiella pneumoniae subsp. pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T181692 NZ_CP086100:477538-477822 [Enterobacter ludwigii]
ATGAGTTATGAACTCGTATTTGATCCGAGGGCGTTCAAGGAGTGGACCAGGCCGGGTGATACCGTAAAAAATCAGTTTAA
GAAGAAGCTTGCGAACGTGTTGGTGAACCCCTGGGTTGAATCGGCTCGCCTGCATGGCCTGCCTGATTGCGACAAGATAA
AACTCAGATCCCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAATGTTGTGACGGTATGTGTTATTACTATCGGTAAG
AGAGAGAAATCAGCCGTTTATCATGACGTAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT181692 WP_032408893.1 NZ_CP064769:163188-163418 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT181692 NZ_CP086100:477306-477548 [Enterobacter ludwigii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTTGAAAAACTCAATATCACGCC
AACGGAAGCCGTTCGGTTACTGTTTCAGTACGTTGCGGAGACGGGGCATATGCCGGTGAAAATGGTCACTGTTAGCGACA
GTGAAGATGAACTCATACAGACAGTCCGGGAGCGGCTGGCTAATCCGCAAAAAGGAATTAAGGTTAGGCTGGATGAGTTA
TGA