Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 139292..140037 | Replicon | plasmid pHS20R14-1 |
| Accession | NZ_CP064769 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain HS20R14 isolate HS20R14 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | A0A331KSM6 |
| Locus tag | HS20R14_RS26720 | Protein ID | WP_032408901.1 |
| Coordinates | 139546..140037 (+) | Length | 164 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | - |
| Locus tag | HS20R14_RS26715 | Protein ID | WP_014386183.1 |
| Coordinates | 139292..139558 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HS20R14_RS26665 (HS20R14_26610) | 134844..135257 | - | 414 | WP_013023817.1 | helix-turn-helix domain-containing protein | - |
| HS20R14_RS26670 (HS20R14_26615) | 135258..135536 | - | 279 | WP_004152721.1 | helix-turn-helix transcriptional regulator | - |
| HS20R14_RS26675 (HS20R14_26620) | 135526..135846 | - | 321 | WP_014386190.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HS20R14_RS26680 (HS20R14_26625) | 135927..136151 | - | 225 | WP_014386189.1 | hypothetical protein | - |
| HS20R14_RS26685 (HS20R14_26630) | 136162..136374 | - | 213 | WP_014386188.1 | hypothetical protein | - |
| HS20R14_RS26690 (HS20R14_26635) | 136436..136762 | - | 327 | WP_014386187.1 | hypothetical protein | - |
| HS20R14_RS26695 (HS20R14_26640) | 137399..137749 | - | 351 | WP_014386186.1 | hypothetical protein | - |
| HS20R14_RS26700 (HS20R14_26645) | 137746..138018 | - | 273 | WP_032408902.1 | hypothetical protein | - |
| HS20R14_RS26705 (HS20R14_26650) | 138208..138693 | + | 486 | WP_014386185.1 | hypothetical protein | - |
| HS20R14_RS26710 (HS20R14_26655) | 138937..139095 | - | 159 | WP_014386184.1 | type I toxin-antitoxin system Hok family toxin | - |
| HS20R14_RS26715 (HS20R14_26660) | 139292..139558 | + | 267 | WP_014386183.1 | DUF1778 domain-containing protein | Antitoxin |
| HS20R14_RS26720 (HS20R14_26665) | 139546..140037 | + | 492 | WP_032408901.1 | GNAT family N-acetyltransferase | Toxin |
| HS20R14_RS26725 (HS20R14_26670) | 140478..140729 | - | 252 | WP_032408900.1 | aldo/keto reductase | - |
| HS20R14_RS26730 (HS20R14_26675) | 140926..142518 | - | 1593 | Protein_163 | IS66 family transposase | - |
| HS20R14_RS26735 (HS20R14_26680) | 142549..142899 | - | 351 | WP_014386180.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| HS20R14_RS26740 (HS20R14_26685) | 142896..143336 | - | 441 | WP_014386179.1 | transposase | - |
| HS20R14_RS26745 (HS20R14_26690) | 143598..144353 | - | 756 | WP_032408898.1 | DeoR/GlpR family DNA-binding transcription regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | mph(A) | - | 1..184047 | 184047 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 164 a.a. Molecular weight: 17667.46 Da Isoelectric Point: 8.8757
>T181691 WP_032408901.1 NZ_CP064769:139546-140037 [Klebsiella pneumoniae subsp. pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT
Download Length: 492 bp
>T181691 NZ_CP086099:c4497594-4497310 [Enterobacter ludwigii]
ATGAGTTATGAACTCGTATTTGATCCGAGGGCGTTCAAGGAGTGGACCAGGCCGGGTGATACCGTAAAAAATCAGTTTAA
GAAGAAGCTTGCGAACGTGTTGGTGAACCCCTGGGTTGAATCGGCTCGCCTGCATGGCCTGCCTGATTGCGACAAGATAA
AACTCAGATCCCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAATGTTGTGACGGTATGTGTTATTACTATCGGTAAG
AGAGAGAAATCAGCCGTTTATCATGACGTAAACAAACGGCTTTAG
ATGAGTTATGAACTCGTATTTGATCCGAGGGCGTTCAAGGAGTGGACCAGGCCGGGTGATACCGTAAAAAATCAGTTTAA
GAAGAAGCTTGCGAACGTGTTGGTGAACCCCTGGGTTGAATCGGCTCGCCTGCATGGCCTGCCTGATTGCGACAAGATAA
AACTCAGATCCCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAATGTTGTGACGGTATGTGTTATTACTATCGGTAAG
AGAGAGAAATCAGCCGTTTATCATGACGTAAACAAACGGCTTTAG
Antitoxin
Download Length: 89 a.a. Molecular weight: 10090.66 Da Isoelectric Point: 5.1169
>AT181691 WP_014386183.1 NZ_CP064769:139292-139558 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK
Download Length: 267 bp
>AT181691 NZ_CP086099:c4497826-4497584 [Enterobacter ludwigii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTTGAAAAACTCAATATCACGCC
AACGGAAGCCGTTCGGTTACTGTTTCAGTACGTTGCGGAGACGGGGCATATGCCGGTGAAAATGGTCACTGTTAGCGACA
GTGAAGATGAACTCATACAGACAGTCCGGGAGCGGCTGGCTAATCCGCAAAAAGGAATTAAGGTTAGGCTGGATGAGTTA
TGA
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTTGAAAAACTCAATATCACGCC
AACGGAAGCCGTTCGGTTACTGTTTCAGTACGTTGCGGAGACGGGGCATATGCCGGTGAAAATGGTCACTGTTAGCGACA
GTGAAGATGAACTCATACAGACAGTCCGGGAGCGGCTGGCTAATCCGCAAAAAGGAATTAAGGTTAGGCTGGATGAGTTA
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|