Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5304126..5304751 Replicon chromosome
Accession NZ_CP064768
Organism Klebsiella pneumoniae subsp. pneumoniae strain HS20R14

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HS20R14_RS25555 Protein ID WP_002882817.1
Coordinates 5304126..5304509 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HS20R14_RS25560 Protein ID WP_004150355.1
Coordinates 5304509..5304751 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS20R14_RS25540 (5301492) 5301492..5302394 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HS20R14_RS25545 (5302391) 5302391..5303026 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HS20R14_RS25550 (5303023) 5303023..5303952 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HS20R14_RS25555 (5304126) 5304126..5304509 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HS20R14_RS25560 (5304509) 5304509..5304751 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HS20R14_RS25565 (5304956) 5304956..5305873 + 918 WP_004181612.1 alpha/beta hydrolase -
HS20R14_RS25570 (5305887) 5305887..5306828 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HS20R14_RS25575 (5306873) 5306873..5307310 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HS20R14_RS25580 (5307307) 5307307..5308167 - 861 WP_004146232.1 virulence factor BrkB family protein -
HS20R14_RS25585 (5308161) 5308161..5308760 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T181689 WP_002882817.1 NZ_CP064768:c5304509-5304126 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T181689 NZ_CP086099:2242764-2242867 [Enterobacter ludwigii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT181689 WP_004150355.1 NZ_CP064768:c5304751-5304509 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT181689 NZ_CP086099:c2242879-2242729 [Enterobacter ludwigii]
TTTATAATAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCT
AAAAGTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References