Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4724873..4725683 Replicon chromosome
Accession NZ_CP064768
Organism Klebsiella pneumoniae subsp. pneumoniae strain HS20R14

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HS20R14_RS22885 Protein ID WP_004178461.1
Coordinates 4724873..4725406 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HS20R14_RS22890 Protein ID WP_002887278.1
Coordinates 4725417..4725683 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS20R14_RS22880 (4723704) 4723704..4724825 + 1122 WP_002887282.1 cupin domain-containing protein -
HS20R14_RS22885 (4724873) 4724873..4725406 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
HS20R14_RS22890 (4725417) 4725417..4725683 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HS20R14_RS22895 (4725786) 4725786..4727219 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
HS20R14_RS22900 (4727209) 4727209..4727892 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HS20R14_RS22905 (4728064) 4728064..4729449 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
HS20R14_RS22910 (4729467) 4729467..4729811 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T181686 WP_004178461.1 NZ_CP064768:c4725406-4724873 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T181686 NZ_CP086069:c4916403-4916155 [Rhodococcus pyridinivorans]
ATGGCTGCCATCACTGCTACCGAAGCGAGAAAAAACTTCTTTCCGCTGCTCGAACAGGTCAACAAGGACCACACCCCTGT
GGAAATCGTATCCAAGCGGGGCAATGCCGTCCTGATGTCGAAGGATGACTACGACGCCATGCTCGAGACCTCGTACCTGA
TGCAGTCTCCGGCCAACGCCCGACGACTGCTCGCCAGCATGCAGAGAGCTCGCGGAGGTGAATACACCGAAAGGGAACTC
ATCGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT181686 WP_002887278.1 NZ_CP064768:c4725683-4725417 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT181686 NZ_CP086069:c4916158-4915904 [Rhodococcus pyridinivorans]
GTGAACATCACCTTCACCGACGAAGCCTGGGACGACTACCAGTTCTGGATCTCGACCGACAGAAGGCAGCTCAAGCGTGT
CAACGAGCTGATCAGGGATTGCCAACGAGACCCGTACTCGGGGATCGGCAAACCCGAACGGTTCAGACATGCCCTGTCAG
GACTGTGGTCTCGACGTATCGACAAAGAACACCGCCTGGTCTACGAGGTCATCGACGGTGCCCTGGTCATCATCGCCGCC
CGCTACCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References