Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4090561..4091180 Replicon chromosome
Accession NZ_CP064768
Organism Klebsiella pneumoniae subsp. pneumoniae strain HS20R14

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HS20R14_RS19880 Protein ID WP_002892050.1
Coordinates 4090962..4091180 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HS20R14_RS19875 Protein ID WP_002892066.1
Coordinates 4090561..4090935 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS20R14_RS19865 (4085713) 4085713..4086906 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HS20R14_RS19870 (4086929) 4086929..4090075 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HS20R14_RS19875 (4090561) 4090561..4090935 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HS20R14_RS19880 (4090962) 4090962..4091180 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HS20R14_RS19885 (4091339) 4091339..4091905 + 567 WP_002892030.1 maltose O-acetyltransferase -
HS20R14_RS19890 (4091877) 4091877..4092017 - 141 WP_004147370.1 hypothetical protein -
HS20R14_RS19895 (4092038) 4092038..4092508 + 471 WP_002892026.1 YlaC family protein -
HS20R14_RS19900 (4092483) 4092483..4093934 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HS20R14_RS19905 (4094035) 4094035..4094733 + 699 WP_002892021.1 GNAT family protein -
HS20R14_RS19910 (4094730) 4094730..4094870 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HS20R14_RS19915 (4094870) 4094870..4095133 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T181685 WP_002892050.1 NZ_CP064768:4090962-4091180 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T181685 NZ_CP086069:c4916158-4915904 [Rhodococcus pyridinivorans]
GTGAACATCACCTTCACCGACGAAGCCTGGGACGACTACCAGTTCTGGATCTCGACCGACAGAAGGCAGCTCAAGCGTGT
CAACGAGCTGATCAGGGATTGCCAACGAGACCCGTACTCGGGGATCGGCAAACCCGAACGGTTCAGACATGCCCTGTCAG
GACTGTGGTCTCGACGTATCGACAAAGAACACCGCCTGGTCTACGAGGTCATCGACGGTGCCCTGGTCATCATCGCCGCC
CGCTACCACTACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT181685 WP_002892066.1 NZ_CP064768:4090561-4090935 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT181685 NZ_CP086069:c4916403-4916155 [Rhodococcus pyridinivorans]
ATGGCTGCCATCACTGCTACCGAAGCGAGAAAAAACTTCTTTCCGCTGCTCGAACAGGTCAACAAGGACCACACCCCTGT
GGAAATCGTATCCAAGCGGGGCAATGCCGTCCTGATGTCGAAGGATGACTACGACGCCATGCTCGAGACCTCGTACCTGA
TGCAGTCTCCGGCCAACGCCCGACGACTGCTCGCCAGCATGCAGAGAGCTCGCGGAGGTGAATACACCGAAAGGGAACTC
ATCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References