Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 845436..846093 Replicon chromosome
Accession NZ_CP064768
Organism Klebsiella pneumoniae subsp. pneumoniae strain HS20R14

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag HS20R14_RS04220 Protein ID WP_002916310.1
Coordinates 845683..846093 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag HS20R14_RS04215 Protein ID WP_002916312.1
Coordinates 845436..845702 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS20R14_RS04190 (840592) 840592..842025 - 1434 WP_004185559.1 6-phospho-beta-glucosidase BglA -
HS20R14_RS04195 (842144) 842144..842872 - 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
HS20R14_RS04200 (842922) 842922..843233 + 312 WP_004144734.1 N(4)-acetylcytidine aminohydrolase -
HS20R14_RS04205 (843397) 843397..844056 + 660 WP_002916317.1 hemolysin III family protein -
HS20R14_RS04210 (844207) 844207..845190 - 984 WP_020324927.1 tRNA-modifying protein YgfZ -
HS20R14_RS04215 (845436) 845436..845702 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
HS20R14_RS04220 (845683) 845683..846093 + 411 WP_002916310.1 protein YgfX Toxin
HS20R14_RS04225 (846100) 846100..846621 - 522 WP_004144730.1 flavodoxin FldB -
HS20R14_RS04230 (846722) 846722..847618 + 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
HS20R14_RS04235 (847641) 847641..848354 + 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HS20R14_RS04240 (848360) 848360..850093 + 1734 WP_004149758.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T181674 WP_002916310.1 NZ_CP064768:845683-846093 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T181674 NZ_CP086064:1451718-1452065 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGTTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT181674 WP_002916312.1 NZ_CP064768:845436-845702 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT181674 NZ_CP086064:1451457-1451708 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACTTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References