181671

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3312505..3313138 Replicon chromosome
Accession NZ_CP064767
Organism Weizmannia coagulans strain BC01

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag IR208_RS15845 Protein ID WP_013860680.1
Coordinates 3312505..3312855 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag IR208_RS15850 Protein ID WP_014096808.1
Coordinates 3312860..3313138 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IR208_RS15810 3307761..3308540 - 780 WP_014096815.1 RNA polymerase sigma factor SigB -
IR208_RS15815 3308518..3308988 - 471 WP_014096814.1 anti-sigma B factor RsbW -
IR208_RS15820 3308992..3309327 - 336 WP_026104587.1 anti-sigma factor antagonist -
IR208_RS15825 3309397..3310407 - 1011 WP_017551130.1 PP2C family protein-serine/threonine phosphatase -
IR208_RS15830 3310421..3310822 - 402 WP_014096811.1 anti-sigma regulatory factor -
IR208_RS15835 3310827..3311183 - 357 WP_014096810.1 STAS domain-containing protein -
IR208_RS15840 3311187..3312014 - 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
IR208_RS15845 3312505..3312855 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IR208_RS15850 3312860..3313138 - 279 WP_014096808.1 hypothetical protein Antitoxin
IR208_RS15855 3313291..3314442 - 1152 WP_014096807.1 alanine racemase -
IR208_RS15860 3314644..3315660 - 1017 WP_014096806.1 outer membrane lipoprotein carrier protein LolA -
IR208_RS15865 3315801..3316151 - 351 WP_014096805.1 holo-ACP synthase -
IR208_RS15870 3316432..3317031 + 600 WP_014096804.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T181671 WP_013860680.1 NZ_CP064767:c3312855-3312505 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T181671 NZ_CP086064:c148244-147963 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT181671 WP_014096808.1 NZ_CP064767:c3313138-3312860 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT181671 NZ_CP086064:c148468-148241 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References