Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1423455..1424032 Replicon chromosome
Accession NZ_CP064760
Organism Microbacterium schleiferi strain A32-1

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag IT882_RS06820 Protein ID WP_195694170.1
Coordinates 1423455..1423802 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag IT882_RS06825 Protein ID WP_195694171.1
Coordinates 1423820..1424032 (-) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IT882_RS06790 1418792..1419328 + 537 WP_195693701.1 hypothetical protein -
IT882_RS06795 1419443..1420195 + 753 WP_195693702.1 DUF2071 domain-containing protein -
IT882_RS06800 1420240..1421826 - 1587 WP_195693703.1 DUF222 domain-containing protein -
IT882_RS06805 1422104..1422865 + 762 WP_195693704.1 hypothetical protein -
IT882_RS06810 1422926..1423189 + 264 WP_195693705.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
IT882_RS06815 1423186..1423446 + 261 WP_195693706.1 type II toxin-antitoxin system RelE/ParE family toxin -
IT882_RS06820 1423455..1423802 - 348 WP_195694170.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IT882_RS06825 1423820..1424032 - 213 WP_195694171.1 ribbon-helix-helix domain-containing protein Antitoxin
IT882_RS06830 1424334..1424918 + 585 WP_195693707.1 hypothetical protein -
IT882_RS06835 1424932..1425882 - 951 WP_195692784.1 DDE-type integrase/transposase/recombinase -
IT882_RS06840 1425879..1426196 - 318 WP_195692783.1 transposase -
IT882_RS06845 1426305..1426961 + 657 WP_195693708.1 hypothetical protein -
IT882_RS06850 1426975..1427271 - 297 WP_195693709.1 restriction endonuclease -
IT882_RS06855 1427318..1428724 - 1407 WP_135950160.1 IS1380 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1427318..1428724 1406


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12019.75 Da        Isoelectric Point: 11.6072

>T181666 WP_195694170.1 NZ_CP064760:c1423802-1423455 [Microbacterium schleiferi]
MRRGHVVLVDLDPAVGSEAARTRPAIVVSNATANLSAARTGHGVITVVPVTSNISTVHPFQVLLSPEQSGLPQPSKAQAE
QVRAVSISRVSRVTGRITAESLGALDDALRLHLSL

Download         Length: 348 bp

>T181666 NZ_CP086061:c2270826-2270575 [Bacillus sp. BC1-43]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGCAAACATGAAACGATTCTCACTATGGTT
TACCCATATCACATTCATTGGCTTATTCTTAATGTTTCAGCTCATCAAAGATTACTTCAGCAGCGAAGGAAAGGCACTAA
TCAACACAATTTTTGTAGTCACATGTATCATTGCCATACTGTTATGGATCATCTATTTTGTATTCCTTAATCTAAGAAAC
AAGTCACACTAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7373.99 Da        Isoelectric Point: 4.0491

>AT181666 WP_195694171.1 NZ_CP064760:c1424032-1423820 [Microbacterium schleiferi]
VKVSLSLSTDDLAFLDAQTRTGVYSSRSAAVQDAVRALREERLADAYADAFAEPGDDAWDAASGDGLARP

Download         Length: 213 bp

>AT181666 NZ_CP086061:2270804-2270904 [Bacillus sp. BC1-43]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References