Detailed information of TA system TA181656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 33453..34063
• Replicon: plasmid p155_2
• Accession: NZ_CP064706
• Organism: Bradyrhizobium sp. 155

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: IVA93_RS39645
• Protein ID: WP_247365381.1
• Coordinates: 33453..33722 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: IVA93_RS39650
• Protein ID: WP_247365166.1
• Coordinates: 33725..34063 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVA93_RS39625 (IVA93_39200)	28998..30761	-	1764	WP_247365178.1	ABC transporter ATP-binding protein/permease	-
IVA93_RS39630	30899..31294	-	396	WP_247365176.1	hypothetical protein	-
IVA93_RS39635	31383..32444	-	1062	WP_247790151.1	outer membrane beta-barrel protein	-
IVA93_RS39640 (IVA93_39210)	32921..33298	+	378	Protein_31	transposase	-
IVA93_RS39645 (IVA93_39215)	33453..33722	+	270	WP_247365381.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IVA93_RS39650 (IVA93_39220)	33725..34063	+	339	WP_247365166.1	helix-turn-helix transcriptional regulator	Antitoxin
IVA93_RS39655 (IVA93_39225)	34876..35046	+	171	WP_247293539.1	hypothetical protein	-
IVA93_RS39660 (IVA93_39230)	35036..37092	+	2057	Protein_35	recombinase family protein	-
IVA93_RS39665 (IVA93_39235)	37007..37462	-	456	WP_247790181.1	hypothetical protein	-
IVA93_RS39670 (IVA93_39240)	37472..38478	-	1007	Protein_37	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..234934	234934
	-	flank	IS/Tn	-	-	32918..33298	380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-73)

Antitoxin

• HigA2-like, helix-turn-helix domain (15-94)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 9942.38 Da
• Isoelectric Point: 9.7795

>T181656 WP_247365381.1 NZ_CP064706:33453-33722 [Bradyrhizobium sp. 155]
VQDGEEPIAAKALRGFGGRAILELVDDFDSKTYRAVYTVRFAGMVYVLPVFQKKAKKGIATSQQDIEPIKTRLRDAEEHY
RALADKGGR

Nucleotide

• Length: 270 bp

>T181656 NZ_CP086016:2350817-2351572 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGACGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAGCAGGCATTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGACGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTCGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGCAGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGCCTGCAGGCAACGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGCGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTACCGGGATCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGAACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12762.98 Da
• Isoelectric Point: 10.6178

>AT181656 WP_247365166.1 NZ_CP064706:33725-34063 [Bradyrhizobium sp. 155]
MTKRKIEVYEGSGNIFADLGLPNPEERLLKSSIVTELHRLIKARNLTQIKAAKLIGISQPDLSHLLRGDYHNYSAERLMK
MLTVFEQRIEIVMRPHRKAGEPGRITFKPVAA

Nucleotide

• Length: 339 bp

>AT181656 NZ_CP086016:2350315-2350683 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGCAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTCGCCGCGCGCGAGCAATTGGG
ACTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGTCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACGCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA