Detailed information of TA system TA181649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 6062630..6063330
• Replicon: chromosome
• Accession: NZ_CP064701
• Organism: Bradyrhizobium sp. 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1X3E9Q3
• Locus tag: IVB07_RS29125
• Protein ID: WP_018454985.1
• Coordinates: 6062905..6063330 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IVB07_RS29120
• Protein ID: WP_018454984.1
• Coordinates: 6062630..6062908 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVB07_RS29100 (IVB07_28825)	6057714..6058616	-	903	WP_018454980.1	sugar ABC transporter permease	-
IVB07_RS29105 (IVB07_28830)	6058616..6059701	-	1086	WP_247363618.1	ABC transporter ATP-binding protein	-
IVB07_RS29110 (IVB07_28835)	6059714..6060853	-	1140	WP_018454982.1	ABC transporter ATP-binding protein	-
IVB07_RS29115 (IVB07_28840)	6060850..6062400	-	1551	WP_085361305.1	glycerol-3-phosphate dehydrogenase	-
IVB07_RS29120 (IVB07_28845)	6062630..6062908	+	279	WP_018454984.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IVB07_RS29125 (IVB07_28850)	6062905..6063330	+	426	WP_018454985.1	type II toxin-antitoxin system VapC family toxin	Toxin
IVB07_RS29130 (IVB07_28855)	6063333..6064175	-	843	WP_018454986.1	DeoR/GlpR family DNA-binding transcription regulator	-
IVB07_RS29135 (IVB07_28860)	6064267..6064941	-	675	WP_018454987.1	HAD-IA family hydrolase	-
IVB07_RS29140 (IVB07_28865)	6065096..6065572	+	477	WP_026232853.1	nuclear transport factor 2 family protein	-
IVB07_RS29145 (IVB07_28870)	6065803..6065994	-	192	WP_018454989.1	hypothetical protein	-
IVB07_RS29150 (IVB07_28875)	6066217..6066627	-	411	WP_018454990.1	acyl-CoA thioesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (13-74)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15519.85 Da
• Isoelectric Point: 5.8803

>T181649 WP_018454985.1 NZ_CP064701:6062905-6063330 [Bradyrhizobium sp. 172]
VNLLLDTNVLSEVQRPAPSPKVLSWLDTIDEDRAFISAASIAELRRGIALLEDGRRRAALAAWLARDLPERFAGRTLPID
HAVAERWGDLMAQSRRSGLALSVMDGFFAATALANNLMLVTRNVKDFAVFGVPLLNPWDNP

Nucleotide

• Length: 426 bp

>T181649 NZ_CP086010:c5589438-5589160 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10060.30 Da
• Isoelectric Point: 6.0741

>AT181649 WP_018454984.1 NZ_CP064701:6062630-6062908 [Bradyrhizobium sp. 172]
MTEHTAPDSPATDSWTLANAKARLSEVIDRAQAGPQIITRHGKPNAVIVSVEEWARKTARKGTLAEFLLASPLRSGDLEL
ERVHDAPHDELP

Nucleotide

• Length: 279 bp

>AT181649 NZ_CP086010:c5589148-5588843 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A1X3E9Q3