Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 4199837..4200468 Replicon chromosome
Accession NZ_CP064692
Organism Bradyrhizobium sp. 191

Toxin (Protein)


Gene name vapC Uniprot ID A0A151FNT4
Locus tag IVB23_RS19500 Protein ID WP_061880318.1
Coordinates 4199837..4200241 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A4R3TKZ8
Locus tag IVB23_RS19505 Protein ID WP_061880347.1
Coordinates 4200238..4200468 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVB23_RS19490 (IVB23_19335) 4196858..4199305 + 2448 WP_247799721.1 adenylate/guanylate cyclase domain-containing protein -
IVB23_RS19495 (IVB23_19340) 4199524..4199742 - 219 WP_061880319.1 hypothetical protein -
IVB23_RS19500 (IVB23_19345) 4199837..4200241 - 405 WP_061880318.1 PIN domain-containing protein Toxin
IVB23_RS19505 (IVB23_19350) 4200238..4200468 - 231 WP_061880347.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IVB23_RS19510 (IVB23_19355) 4200761..4201468 + 708 WP_245312060.1 GntR family transcriptional regulator -
IVB23_RS19515 (IVB23_19360) 4201516..4202478 + 963 WP_247799722.1 ABC transporter substrate-binding protein -
IVB23_RS19520 (IVB23_19365) 4202485..4203240 + 756 WP_247799723.1 ABC transporter permease -
IVB23_RS19525 (IVB23_19370) 4203219..4204025 + 807 WP_247799724.1 ABC transporter ATP-binding protein -
IVB23_RS19530 (IVB23_19375) 4204033..4204842 + 810 WP_247799725.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-114)

Antitoxin

SpoVT-AbrB domain

(5-36)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14738.77 Da        Isoelectric Point: 6.7156

>T181643 WP_061880318.1 NZ_CP064692:c4200241-4199837 [Bradyrhizobium sp. 191]
VRPFFDSNILIYAYSTDIRRQRALTMIAGGGVISAQVLNEFTNVLRKKQKQDWPVIEAAVQSLRFRFPDIAPLTSDTHTA
ALALARDHNLAFYDALIVAAAIEAGCDTLYSEDLQHGRSFSGLTIVNPFPGSTP

Download         Length: 405 bp

>T181643 NZ_CP086010:1626351-1627220 [Pseudomonas aeruginosa]
ATGAACATGGCGCAGACCCACACCCCCGTCCCGAACCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTCCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGTTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCGTGCCTGGAGCTGCTGCTGGATGCGCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGCCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCTTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCTCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGTCGATTCCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8726.87 Da        Isoelectric Point: 4.8232

>AT181643 WP_061880347.1 NZ_CP064692:c4200468-4200238 [Bradyrhizobium sp. 191]
MLVSKWGNSLAVRLPKALVDALNLSPGDELNVVEASKDRLAVEKIDKRAEFLRQAEQFHFPLPDDYKFDRDEANER

Download         Length: 231 bp

>AT181643 NZ_CP086010:1627213-1628046 [Pseudomonas aeruginosa]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCGCGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACCCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTTTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAGCTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTCGCGGCGCCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCCGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTGCGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A151FNT4

Antitoxin

Source ID Structure
AlphaFold DB A0A4R3TKZ8

References