Detailed information of TA system TA181642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 3071202..3072732
• Replicon: chromosome
• Accession: NZ_CP064692
• Organism: Bradyrhizobium sp. 191

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: IVB23_RS14105
• Protein ID: WP_247320960.1
• Coordinates: 3071202..3072122 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: IVB23_RS14110
• Protein ID: WP_247320962.1
• Coordinates: 3072112..3072732 (-)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVB23_RS14095 (IVB23_14010)	3069717..3070949	-	1233	WP_247803937.1	exonuclease SbcCD subunit D	-
IVB23_RS14100 (IVB23_14015)	3070946..3071128	-	183	WP_247320958.1	hypothetical protein	-
IVB23_RS14105 (IVB23_14020)	3071202..3072122	-	921	WP_247320960.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
IVB23_RS14110 (IVB23_14025)	3072112..3072732	-	621	WP_247320962.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
IVB23_RS14115 (IVB23_14030)	3072942..3073640	-	699	WP_247803938.1	DCL family protein	-
IVB23_RS14120 (IVB23_14035)	3073661..3076144	-	2484	WP_247803939.1	AAA family ATPase	-
IVB23_RS14125 (IVB23_14040)	3076137..3076301	-	165	WP_247803940.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-259)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (12-56)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 33714.73 Da
• Isoelectric Point: 4.7975

>T181642 WP_247320960.1 NZ_CP064692:c3072122-3071202 [Bradyrhizobium sp. 191]
MAREPRNIGASVRARLLDRARAERSDFQILLTRYALERLLYRLSVSPHRDRFILKGAMLFVTWVADPFRPTRDLDLLGNG
DSDAEAIAETFRAICAQPVADDGVAFDVAALMAAPIREEVEYGGVRVRTTATIAGARIPIQVDIGFGDAITPAAVEIDYP
TLLDAPTPHLRAYPVETVVAEKFEALVTLGVANSRLKDFYDLWVISRTFELRRAALVEAIQRTFERRGTVLPSVVPVGLT
DEFAEASAAQWRAFLGRDRMAAAPDVFAIAVADLRLFLMPLVVGLEEERIWPPSGPWSPGAAIDEA

Nucleotide

• Length: 921 bp

>T181642 NZ_CP086010:c144876-144595 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 23333.29 Da
• Isoelectric Point: 10.3406

>AT181642 WP_247320962.1 NZ_CP064692:c3072732-3072112 [Bradyrhizobium sp. 191]
MPSTSGQRLRALDLLKTRGMLRLKDFVAEGIGPETLARLVREEVVVRPARGLYQLPDAHVEAAHTLAEAAVLVPKGVVCL
TSALQYHELTLQMPSAVWMAIDRVAWRPKIDYPAIRFVRFTGSALTEGVERHRIENVDVPITNPAKTIVDCFRYRTKIGL
DVAMEGLREGLRRRRCKPDQLWRYARKARVWSVMRPYVEAMVSDGA

Nucleotide

• Length: 621 bp

>AT181642 NZ_CP086010:c145100-144873 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA