Detailed information of TA system TA181638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 7014316..7014856
• Replicon: chromosome
• Accession: NZ_CP064690
• Organism: Bradyrhizobium sp. 192

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: IVB24_RS32900
• Protein ID: WP_094977258.1
• Coordinates: 7014596..7014856 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: IVB24_RS32895
• Protein ID: WP_094977259.1
• Coordinates: 7014316..7014609 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVB24_RS32870 (IVB24_32560)	7010135..7010992	-	858	WP_247770501.1	3-mercaptopyruvate sulfurtransferase	-
IVB24_RS32875 (IVB24_32565)	7011153..7011566	-	414	WP_247770502.1	rhodanese-like domain-containing protein	-
IVB24_RS32880 (IVB24_32570)	7011719..7012894	-	1176	WP_247770503.1	VWA domain-containing protein	-
IVB24_RS32885 (IVB24_32575)	7013018..7013263	+	246	WP_018318587.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
IVB24_RS32890 (IVB24_32580)	7013359..7014201	+	843	WP_027529387.1	MoxR family ATPase	-
IVB24_RS32895 (IVB24_32585)	7014316..7014609	-	294	WP_094977259.1	BrnA antitoxin family protein	Antitoxin
IVB24_RS32900 (IVB24_32590)	7014596..7014856	-	261	WP_094977258.1	BrnT family toxin	Toxin
IVB24_RS32905 (IVB24_32595)	7015040..7016686	-	1647	WP_247770504.1	GMC family oxidoreductase N-terminal domain-containing protein	-
IVB24_RS32910 (IVB24_32600)	7016704..7017297	-	594	WP_247770505.1	GNAT family protein	-
IVB24_RS32915 (IVB24_32605)	7017457..7018383	-	927	WP_247770506.1	SMP-30/gluconolactonase/LRE family protein	-
IVB24_RS32920 (IVB24_32610)	7018633..7019736	+	1104	WP_247770507.1	mandelate racemase/muconate lactonizing enzyme family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-92)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9864.46 Da
• Isoelectric Point: 10.5508

>T181638 WP_094977258.1 NZ_CP064690:c7014856-7014596 [Bradyrhizobium sp. 192]
MKIVWDEPKRLANLDKHELDFADLNETFFDNALVIPSHNKSKRWVAIGVSIRGVIVVVFARLGREGVSIISMRPASRSER
KLYAER

Nucleotide

• Length: 261 bp

>T181638 NZ_CP085987:c4334732-4334334 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10779.42 Da
• Isoelectric Point: 11.3548

>AT181638 WP_094977259.1 NZ_CP064690:c7014609-7014316 [Bradyrhizobium sp. 192]
MPNAKKKSGYTKRDLRAVSDTPELTRADFAKARPFSEVFPDLSAAIRKGRGPNKAPTKTLVSLRLSPEVVEHFKSTGPGW
QSRIDETLRKAVKRKAS

Nucleotide

• Length: 294 bp

>AT181638 NZ_CP085987:c4334959-4334732 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA