Detailed information of TA system TA181627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 711243..711842
• Replicon: chromosome
• Accession: NZ_CP064686
• Organism: Bradyrhizobium sp. 4

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: IVB45_RS03370
• Protein ID: WP_247363185.1
• Coordinates: 711546..711842 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H5YDN2
• Locus tag: IVB45_RS03365
• Protein ID: WP_007605286.1
• Coordinates: 711243..711542 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVB45_RS03345 (IVB45_03335)	707140..707571	-	432	WP_247283456.1	thioesterase family protein	-
IVB45_RS03350 (IVB45_03340)	707582..708343	-	762	WP_027570150.1	SDR family NAD(P)-dependent oxidoreductase	-
IVB45_RS03355 (IVB45_03345)	708369..710255	-	1887	WP_247362853.1	feruloyl-CoA synthase	-
IVB45_RS03360 (IVB45_03350)	710252..711061	-	810	WP_247362851.1	crotonase/enoyl-CoA hydratase family protein	-
IVB45_RS03365 (IVB45_03355)	711243..711542	-	300	WP_007605286.1	putative addiction module antidote protein	Antitoxin
IVB45_RS03370 (IVB45_03360)	711546..711842	-	297	WP_247363185.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IVB45_RS03375 (IVB45_03365)	711909..712370	-	462	WP_247362847.1	DUF3237 domain-containing protein	-
IVB45_RS03380 (IVB45_03370)	712382..713701	-	1320	WP_027515392.1	TRAP transporter permease	-
IVB45_RS03385 (IVB45_03375)	713704..714213	-	510	WP_007599673.1	TRAP transporter small permease	-
IVB45_RS03390 (IVB45_03380)	714340..715356	-	1017	WP_247362846.1	TRAP transporter substrate-binding protein	-
IVB45_RS03395 (IVB45_03385)	715571..716740	+	1170	WP_247362845.1	4-hydroxybenzoate 3-monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-82)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10691.32 Da
• Isoelectric Point: 9.5713

>T181627 WP_247363185.1 NZ_CP064686:c711842-711546 [Bradyrhizobium sp. 4]
IPTIKRTDEFSDWLRKQRDRRAKAKILARIDRLALGNPGDVAAVGGGISEMRVHEGAGYRIYYVARGEEIIVLLCGGDKG
SQPGDIALAKKIASELEV

Nucleotide

• Length: 297 bp

>T181627 NZ_CP085987:1813650-1813868 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10334.80 Da
• Isoelectric Point: 4.7513

>AT181627 WP_007605286.1 NZ_CP064686:c711542-711243 [Bradyrhizobium sp. 4]
MAKVKSFDAAEYLDSPEMIAAYLTEAFESDDPSAITMAIGAVARSQGMGAIAEKAGLSRENLYRSLGGEAKPEFGTVIKV
LHAMGINLVAQPQSGTKAA

Nucleotide

• Length: 300 bp

>AT181627 NZ_CP085987:1813248-1813622 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: H5YDN2