181585

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 259039..259718 Replicon chromosome
Accession NZ_CP064682
Organism Escherichia coli K-12 strain BZKR2G40

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag IVK99_RS01230 Protein ID WP_000854672.1
Coordinates 259039..259380 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag IVK99_RS01235 Protein ID WP_000070395.1
Coordinates 259401..259718 (-) Length 106 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVK99_RS01205 254316..254717 + 402 WP_000174689.1 sigma factor-binding protein Crl -
IVK99_RS01210 254756..255811 - 1056 WP_000749863.1 phosphoporin PhoE -
IVK99_RS01215 256099..257202 + 1104 WP_001285288.1 glutamate 5-kinase -
IVK99_RS01220 257214..258467 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
IVK99_RS01230 259039..259380 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
IVK99_RS01235 259401..259718 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
IVK99_RS01240 259737..259958 - 222 WP_000691994.1 DUF987 domain-containing protein -
IVK99_RS01245 259967..260443 - 477 WP_000811693.1 RadC family protein -
IVK99_RS01250 260459..260917 - 459 WP_000211838.1 antirestriction protein -
IVK99_RS01255 261015..261254 - 240 WP_000194654.1 DUF905 domain-containing protein -
IVK99_RS01260 261331..261798 - 468 WP_001547765.1 hypothetical protein -
IVK99_RS01265 261821..262264 - 444 WP_000824223.1 hypothetical protein -
IVK99_RS01270 262264..262491 - 228 WP_001548158.1 hypothetical protein -
IVK99_RS01275 262487..262678 - 192 Protein_246 DeoR family transcriptional regulator -
IVK99_RS01280 262895..263716 - 822 WP_000197389.1 DUF945 domain-containing protein -
IVK99_RS01285 263808..264671 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(6-104)


Sequences


Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T181585 WP_000854672.1 NZ_CP064682:c259380-259039 [Escherichia coli K-12]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T181585 NZ_CP085977:1344785-1345156 [Mycobacterium avium subsp. avium]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGCTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT181585 WP_000070395.1 NZ_CP064682:c259718-259401 [Escherichia coli K-12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT181585 NZ_CP085977:1344592-1344798 [Mycobacterium avium subsp. avium]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGACACCCGCCGATGATTCTCGTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB P77692


Antitoxin

Source ID Structure
AlphaFold DB Q47684

References