Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 475546..476164 Replicon chromosome
Accession NZ_CP064681
Organism Escherichia coli K-12 strain BZKR3G40

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IVL00_RS02335 Protein ID WP_001291435.1
Coordinates 475546..475764 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IVL00_RS02340 Protein ID WP_000344800.1
Coordinates 475790..476164 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL00_RS02300 470835..471407 + 573 WP_000779842.1 YbaY family lipoprotein -
IVL00_RS02305 471438..471749 - 312 WP_000409911.1 MGMT family protein -
IVL00_RS02315 472128..472481 + 354 WP_000878140.1 DUF1428 domain-containing protein -
IVL00_RS02320 472523..474073 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IVL00_RS02325 474237..474707 - 471 WP_000136192.1 YlaC family protein -
IVL00_RS02330 474823..475374 - 552 WP_000102564.1 maltose O-acetyltransferase -
IVL00_RS02335 475546..475764 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IVL00_RS02340 475790..476164 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IVL00_RS02345 476710..479859 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IVL00_RS02350 479882..481075 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T181543 WP_001291435.1 NZ_CP064681:c475764-475546 [Escherichia coli K-12]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T181543 NZ_CP085952:c408651-408268 [Haemophilus influenzae]
ATGAAACAAGAATGGGAAGTAATTTTACAAGACCCACTTTTGAACTGGTTAGAAACGCTGGCAGAAGATGACGTATTAAA
AATCTATGCGGCGTTGGAATTATTATCAACAGAAGGTCCACAATTAAGCAGACCTTATGCGGATACGCTGCAAGGCTCTA
AATATACCAATTTAAAAGAATTGCGAGTACAGTCTAAATTATCGGTATTCCGTTTATTTTATATTTTCGACCCTGTCAGA
CAAGCGATTGTTTTATGTGGCGGAGATAAAAAAGGCAAGAAAGAAAAACTCTTTTACAAAGAGATGATTGCCCTAGCGGA
ACAAACCTATGATGATTACCTTTCTGAATTAACTAAGGAGCAAGAAAATGAGCGTGAAATTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT181543 WP_000344800.1 NZ_CP064681:c476164-475790 [Escherichia coli K-12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT181543 NZ_CP085952:c408284-407982 [Haemophilus influenzae]
ATGAGCGTGAAATTTAAAGATCTGATGAATAATCTTCCAGCTGAAAAGCAAGCGAAAGTGAAAGCTATGGCAGACGATAT
GCGAATGGAGTTACAACTTTACCGTATTCGTGAAGAATTAGAGCTTTCACAAAAGCAAATGGCAGAAGCATTAAGTATTT
CTCAGCCGTCAGTTGTCGCCCTTGAAAAACGTGGCAATGACATTAAATTATCATCAGTTAAACGTTACATTGAAGCAATG
GGTGGTGTGTTAAATTTATCGGTTGAATTACCCACAGGAAAAACAGTTACTTTCAACTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References