Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3033773..3034427 Replicon chromosome
Accession NZ_CP064680
Organism Escherichia coli K-12 strain CETR1G40

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IVL01_RS14725 Protein ID WP_000244777.1
Coordinates 3033773..3034180 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IVL01_RS14730 Protein ID WP_000354046.1
Coordinates 3034161..3034427 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL01_RS14705 3029730..3031463 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
IVL01_RS14710 3031469..3032179 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IVL01_RS14715 3032204..3033100 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IVL01_RS14720 3033212..3033733 + 522 WP_001055874.1 flavodoxin FldB -
IVL01_RS14725 3033773..3034180 - 408 WP_000244777.1 toxin CptA Toxin
IVL01_RS14730 3034161..3034427 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IVL01_RS14735 3034670..3035650 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IVL01_RS14740 3035846..3036505 - 660 WP_000250274.1 hemolysin III family protein -
IVL01_RS14745 3036669..3036980 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IVL01_RS14750 3037025..3038458 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
IVL01_RS14755 3038515..3039258 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T181527 WP_000244777.1 NZ_CP064680:c3034180-3033773 [Escherichia coli K-12]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T181527 NZ_CP085945:c4853286-4852825 [Mycobacterium intracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCGCTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCGCTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGGCGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT181527 WP_000354046.1 NZ_CP064680:c3034427-3034161 [Escherichia coli K-12]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT181527 NZ_CP085945:c4853478-4853290 [Mycobacterium intracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTCGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References