Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2770472..2771139 Replicon chromosome
Accession NZ_CP064680
Organism Escherichia coli K-12 strain CETR1G40

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag IVL01_RS13510 Protein ID WP_001094400.1
Coordinates 2770810..2771139 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag IVL01_RS13505 Protein ID WP_000072690.1
Coordinates 2770472..2770789 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL01_RS13475 2765524..2766533 - 1010 Protein_2634 arsenic transporter -
IVL01_RS13480 2766675..2768378 + 1704 WP_000896263.1 hypothetical protein -
IVL01_RS13485 2768950..2769173 + 224 Protein_2636 DUF905 family protein -
IVL01_RS13490 2769276..2769734 + 459 WP_000211841.1 antirestriction protein -
IVL01_RS13495 2769743..2770225 + 483 WP_001407480.1 RadC family protein -
IVL01_RS13500 2770234..2770434 + 201 WP_001395452.1 DUF987 domain-containing protein -
IVL01_RS13505 2770472..2770789 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
IVL01_RS13510 2770810..2771139 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
IVL01_RS13515 2771503..2776083 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T181525 WP_001094400.1 NZ_CP064680:2770810-2771139 [Escherichia coli K-12]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T181525 NZ_CP085945:c3495731-3495324 [Mycobacterium intracellulare]
ATGAGCAACCCGCAGGTGGCCGGAGTGCTCGACACTTCGGTGTTCATCGCGACCGAAAGTGGTCGGCCTCTCGACGCGGC
GCTGATCCCTGATCAAGTTGGCACGACGGTTGTTACCCTCGCGGAACTGCACGTTGGTGTGTTGGCCGCGTCGACTTCCG
ATATTAGAGCGCAGCGCCTCGCGACATTGGAATCCATTGCGGATATCGAAGCGCTGCCGATCGATGACGATGCGGCCCGG
ATGTGGGCCCGGCTGCGGATCCATCTCGCCGAGACCGGTCAACGCGTCCGGATCAACGATCTGTGGATCGCGGCCATCGC
GGCGTCCCGAGGGCTACCCGTTGTCACGCAGGACGATGACTTTGCCGTCTTGGATGGTGCGGCCAATTTGACCGTTATCC
GCGTCTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT181525 WP_000072690.1 NZ_CP064680:2770472-2770789 [Escherichia coli K-12]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT181525 NZ_CP085945:c3495988-3495728 [Mycobacterium intracellulare]
ATGTCTGAAGTGGCATCTCGTGAGCTGCGCAACGATACGGCTGGGGTGCTGCGGCGGGTTCGGGATGGAGAGGACGTCAC
CATCACTGTCAGTGGCCGACCGGTCGCAGTTCTTACCGCAGTTGTCCCTCAGCGCAGGCGTTGGCTGAGCAAGACGGAGT
TGCTGTCGCGCTTGCCCCGCGTTCAAGCGGATCCGGGATTACGCGAGGACCTTCACGCACTCGCCGGCGACACTACCGAG
GAGCTGGGCCCGATCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References