Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2070591..2071422 Replicon chromosome
Accession NZ_CP064680
Organism Escherichia coli K-12 strain CETR1G40

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag IVL01_RS10235 Protein ID WP_000854814.1
Coordinates 2071048..2071422 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag IVL01_RS10230 Protein ID WP_001285584.1
Coordinates 2070591..2070959 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL01_RS10215 2068258..2069790 + 1533 WP_001350525.1 hypothetical protein -
IVL01_RS10220 2069787..2070233 + 447 WP_000187523.1 RadC family protein -
IVL01_RS10225 2070296..2070517 + 222 WP_000692323.1 DUF987 domain-containing protein -
IVL01_RS10230 2070591..2070959 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
IVL01_RS10235 2071048..2071422 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
IVL01_RS10240 2071419..2071613 + 195 WP_000988600.1 hypothetical protein -
IVL01_RS10245 2071626..2071739 + 114 WP_001161660.1 DUF957 domain-containing protein -
IVL01_RS10250 2072028..2072156 - 129 Protein_2002 transposase domain-containing protein -
IVL01_RS10255 2072228..2072410 + 183 WP_001016348.1 hypothetical protein -
IVL01_RS10260 2072511..2072840 - 330 WP_000450409.1 DUF496 family protein -
IVL01_RS10265 2073012..2074070 - 1059 WP_001200891.1 FUSC family protein -
IVL01_RS10270 2074268..2074741 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
IVL01_RS10275 2074860..2076026 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T181522 WP_000854814.1 NZ_CP064680:2071048-2071422 [Escherichia coli K-12]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T181522 NZ_CP085923:c548676-548416 [Helicobacter pylori]
GTGTTGAAACTCAACCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAAGAAAGAAAGAACCGCTACCCCTACCATTTAAAGATCACGCTTTAAAGGGAGTTTGGAAGCCTT
ATAGAGAGTGCCACATCAAAGCTGATATTTTGCTTGTGTATTTAGTGCAAGATGATGAGCTGATTTTGTTAAGGTTAGGC
AGTCATAGCGAGTTGTTTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT181522 WP_001285584.1 NZ_CP064680:2070591-2070959 [Escherichia coli K-12]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT181522 NZ_CP085923:c549034-548657 [Helicobacter pylori]
ATGCCAAACACCACCGCAGAAAAAGACTACACCCTATACAGCGAAAAACAGCTTTTTAATTTAATCCATCAATTAGAGCG
AAAAATCAAAAAGATGCAAAATGATAGAGTTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACATTCAAACGCAACAAGCCATGAGAGATGCGCTCAACAAAGAGACAGATTTGATTGTTGAGGA
TTTTTCTAGTTACAGCGATGAAAGAAAAAGGGCTTTGGGTGTTGAAACTCAACCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References