Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1503543..1504181 Replicon chromosome
Accession NZ_CP064680
Organism Escherichia coli K-12 strain CETR1G40

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IVL01_RS07350 Protein ID WP_000813794.1
Coordinates 1503543..1503719 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IVL01_RS07355 Protein ID WP_001270286.1
Coordinates 1503765..1504181 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL01_RS07330 1499162..1500376 - 1215 WP_001326689.1 BenE family transporter YdcO -
IVL01_RS07335 1500429..1500965 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
IVL01_RS07340 1501038..1502999 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IVL01_RS07345 1503091..1503321 - 231 WP_000494244.1 YncJ family protein -
IVL01_RS07350 1503543..1503719 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IVL01_RS07355 1503765..1504181 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IVL01_RS07360 1504260..1505666 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
IVL01_RS07365 1505911..1507056 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
IVL01_RS07370 1507074..1508087 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
IVL01_RS07375 1508088..1509029 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T181511 WP_000813794.1 NZ_CP064680:1503543-1503719 [Escherichia coli K-12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T181511 NZ_CP085898:15992-16342 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT181511 WP_001270286.1 NZ_CP064680:1503765-1504181 [Escherichia coli K-12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT181511 NZ_CP085898:15735-15998 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References