Detailed information of TA system TA181288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 32135..32772
• Replicon: plasmid p3804_4
• Accession: NZ_CP064659
• Organism: Enterobacter hormaechei strain 3804

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A9E6WXZ4
• Locus tag: IT786_RS24095
• Protein ID: WP_032662169.1
• Coordinates: 32135..32545 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A9E6WTF5
• Locus tag: IT786_RS24100
• Protein ID: WP_032662167.1
• Coordinates: 32542..32772 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT786_RS24075	27501..27851	+	351	WP_032662182.1	DUF305 domain-containing protein	-
IT786_RS24080	28037..28945	-	909	WP_032662179.1	HNH endonuclease	-
IT786_RS24085	29492..30514	-	1023	WP_032662174.1	helicase UvrD	-
IT786_RS24090	30499..32061	-	1563	WP_032662171.1	AAA family ATPase	-
IT786_RS24095	32135..32545	-	411	WP_032662169.1	PIN domain-containing protein	Toxin
IT786_RS24100	32542..32772	-	231	WP_032662167.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IT786_RS24105	33314..33604	+	291	WP_013087126.1	hypothetical protein	-
IT786_RS24110	33735..34265	+	531	WP_013087127.1	hypothetical protein	-
IT786_RS24115	34284..35060	+	777	WP_015572054.1	site-specific integrase	-
IT786_RS24120	35262..35519	+	258	Protein_36	site-specific integrase	-
IT786_RS24125	35763..36773	-	1011	WP_015572055.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(D) / qnrA1 / sul1 / catA2 / sul2 / aph(3'')-Ib / aph(6)-Id / qnrS1 / blaTEM-1B	-	1..90483	90483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14729.03 Da
• Isoelectric Point: 5.7351

>T181288 WP_032662169.1 NZ_CP064659:c32545-32135 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 411 bp

>T181288 NZ_CP085817:2457388-2457491 [Salmonella enterica subsp. enterica serovar Enteritidis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8645.77 Da
• Isoelectric Point: 4.4692

>AT181288 WP_032662167.1 NZ_CP064659:c32772-32542 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT181288 NZ_CP085817:c2457493-2457348 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG