Detailed information of TA system TA181286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 212167..212693
• Replicon: plasmid p3804_mcr
• Accession: NZ_CP064657
• Organism: Enterobacter hormaechei strain 3804

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: IT786_RS23060
• Protein ID: WP_000323025.1
• Coordinates: 212167..212454 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: IT786_RS23065
• Protein ID: WP_000534858.1
• Coordinates: 212454..212693 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT786_RS23025	207737..207913	-	177	WP_001371930.1	hypothetical protein	-
IT786_RS23030	208424..209368	+	945	WP_000778029.1	DUF5417 domain-containing protein	-
IT786_RS23035	209467..210066	+	600	WP_001031420.1	hypothetical protein	-
IT786_RS23040	210126..210476	+	351	WP_000743059.1	hypothetical protein	-
IT786_RS23045	210523..210726	+	204	WP_001015183.1	hypothetical protein	-
IT786_RS23050	211008..211328	+	321	WP_000332796.1	hypothetical protein	-
IT786_RS23055	211937..212095	-	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
IT786_RS23060	212167..212454	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
IT786_RS23065	212454..212693	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
IT786_RS23070	212956..213879	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
IT786_RS23075	214079..214651	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
IT786_RS23080	215127..216365	-	1239	WP_000219087.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / blaCTX-M-9 / mcr-9	-	1..267745	267745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T181286 WP_000323025.1 NZ_CP064657:c212454-212167 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T181286 NZ_CP085817:c1548520-1548263 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT181286 WP_000534858.1 NZ_CP064657:c212693-212454 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT181286 NZ_CP085817:1548345-1548677 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8