Detailed information of TA system TA181285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 94275..95898
• Replicon: plasmid p3804_mcr
• Accession: NZ_CP064657
• Organism: Enterobacter hormaechei strain 3804

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: IT786_RS22420
• Protein ID: WP_000088044.1
• Coordinates: 94573..95898 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: IT786_RS22415
• Protein ID: WP_012477377.1
• Coordinates: 94275..94568 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT786_RS22395	90074..90466	+	393	WP_000341066.1	hypothetical protein	-
IT786_RS22400	91125..91781	+	657	WP_000462754.1	trypsin-like serine protease	-
IT786_RS22405	91984..92481	+	498	WP_000340139.1	membrane protein	-
IT786_RS22410	92486..93874	+	1389	WP_012695445.1	hypothetical protein	-
IT786_RS22415	94275..94568	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
IT786_RS22420	94573..95898	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
IT786_RS22425	95959..96165	+	207	WP_000134171.1	hypothetical protein	-
IT786_RS22430	96266..96676	+	411	WP_000985909.1	hypothetical protein	-
IT786_RS22435	96689..97117	+	429	Protein_92	hypothetical protein	-
IT786_RS22440	97395..98399	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / blaCTX-M-9 / mcr-9	-	1..267745	267745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T181285 WP_000088044.1 NZ_CP064657:94573-95898 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T181285 NZ_CP085817:1163178-1163465 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT181285 WP_012477377.1 NZ_CP064657:94275-94568 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT181285 NZ_CP085817:1163452-1163754 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7