Detailed information of TA system TA181272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 302809..303405
• Replicon: chromosome
• Accession: NZ_CP064656
• Organism: Enterobacter hormaechei strain 3804

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: IT786_RS01445
• Protein ID: WP_023315213.1
• Coordinates: 303103..303405 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IT786_RS01440
• Protein ID: WP_023315212.1
• Coordinates: 302809..303096 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT786_RS01435	301181..302812	+	1632	WP_058652456.1	Na/Pi cotransporter family protein	-
IT786_RS01440	302809..303096	-	288	WP_023315212.1	putative addiction module antidote protein	Antitoxin
IT786_RS01445	303103..303405	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IT786_RS01450	303603..304475	+	873	WP_045346556.1	23S rRNA pseudouridine(2604) synthase RluF	-
IT786_RS01455	304476..304748	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
IT786_RS01460	304799..305743	-	945	WP_058652454.1	ketopantoate/pantoate/pantothenate transporter PanS	-
IT786_RS01465	305837..307186	-	1350	WP_003860344.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T181272 WP_023315213.1 NZ_CP064656:c303405-303103 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T181272 NZ_CP085816:c4057257-4056946 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10171.73 Da
• Isoelectric Point: 7.0086

>AT181272 WP_023315212.1 NZ_CP064656:c303096-302809 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLRLTIKPSGD

Nucleotide

• Length: 288 bp

>AT181272 NZ_CP085816:c4056945-4056655 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A837F9W8