Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 512883..513454 Replicon chromosome
Accession NZ_CP064424
Organism Enterococcus faecium strain PR01996-12

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag IUH71_RS02445 Protein ID WP_002286801.1
Coordinates 513113..513454 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag IUH71_RS02440 Protein ID WP_002323011.1
Coordinates 512883..513113 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUH71_RS02410 (IUH71_02410) 508016..509347 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
IUH71_RS02415 (IUH71_02415) 509369..509995 + 627 WP_002286816.1 cysteine hydrolase -
IUH71_RS02420 (IUH71_02420) 510178..510759 + 582 WP_010729547.1 TetR/AcrR family transcriptional regulator -
IUH71_RS15585 511491..512053 + 563 Protein_481 SOS response-associated peptidase family protein -
IUH71_RS02435 (IUH71_02435) 512258..512596 - 339 WP_002286804.1 hypothetical protein -
IUH71_RS02440 (IUH71_02440) 512883..513113 + 231 WP_002323011.1 hypothetical protein Antitoxin
IUH71_RS02445 (IUH71_02445) 513113..513454 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IUH71_RS02450 (IUH71_02450) 514304..514489 + 186 WP_002304207.1 hypothetical protein -
IUH71_RS02455 (IUH71_02455) 514994..515188 + 195 WP_002297028.1 hypothetical protein -
IUH71_RS02460 (IUH71_02460) 515379..515628 + 250 Protein_487 transposase -
IUH71_RS02465 (IUH71_02465) 515872..516702 - 831 WP_002286789.1 manganese catalase family protein -
IUH71_RS02470 (IUH71_02470) 516910..518244 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T181265 WP_002286801.1 NZ_CP064424:513113-513454 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T181265 NZ_CP085816:c2261491-2261388 [Salmonella enterica subsp. enterica serovar Enteritidis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT181265 WP_002323011.1 NZ_CP064424:512883-513113 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT181265 NZ_CP085816:2261386-2261531 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References