Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 450859..451430 Replicon chromosome
Accession NZ_CP064406
Organism Enterococcus faecium strain PR05720-3

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag IUH68_RS02170 Protein ID WP_002286801.1
Coordinates 451089..451430 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag IUH68_RS02165 Protein ID WP_002323011.1
Coordinates 450859..451089 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUH68_RS02140 (IUH68_02140) 446340..447668 + 1329 WP_002327434.1 FAD-containing oxidoreductase -
IUH68_RS02145 (IUH68_02145) 447690..448316 + 627 WP_002286816.1 cysteine hydrolase -
IUH68_RS02150 (IUH68_02150) 448499..449080 + 582 WP_038809688.1 TetR/AcrR family transcriptional regulator -
IUH68_RS02155 (IUH68_02155) 449445..450020 + 576 WP_002352505.1 SOS response-associated peptidase family protein -
IUH68_RS02160 (IUH68_02160) 450225..450563 - 339 WP_002306002.1 hypothetical protein -
IUH68_RS02165 (IUH68_02165) 450859..451089 + 231 WP_002323011.1 hypothetical protein Antitoxin
IUH68_RS02170 (IUH68_02170) 451089..451430 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IUH68_RS02175 (IUH68_02175) 452280..452465 + 186 WP_002304207.1 hypothetical protein -
IUH68_RS02180 (IUH68_02180) 452735..453897 + 1163 WP_212462989.1 IS3 family transposase -
IUH68_RS14490 454028..454270 + 243 Protein_431 LPXTG cell wall anchor domain-containing protein -
IUH68_RS02190 (IUH68_02190) 454344..455240 + 897 WP_002352497.1 class C sortase -
IUH68_RS02195 (IUH68_02195) 455421..456095 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T181263 WP_002286801.1 NZ_CP064406:451089-451430 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T181263 NZ_CP085816:c523284-522757 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT181263 WP_002323011.1 NZ_CP064406:450859-451089 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT181263 NZ_CP085816:c523571-523281 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References