Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3130125..3130812 Replicon chromosome
Accession NZ_CP064405
Organism Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FBY8
Locus tag IRY56_RS14675 Protein ID WP_011799298.1
Coordinates 3130369..3130812 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag IRY56_RS14670 Protein ID WP_003414620.1
Coordinates 3130125..3130382 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRY56_RS14650 3125445..3126299 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
IRY56_RS14655 3126355..3127518 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
IRY56_RS14660 3127535..3128749 - 1215 WP_011799297.1 zinc metalloprotease Rip -
IRY56_RS14665 3128757..3129998 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
IRY56_RS14670 3130125..3130382 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
IRY56_RS14675 3130369..3130812 + 444 WP_011799298.1 type II toxin-antitoxin system VapC family toxin Toxin
IRY56_RS14680 3130892..3131554 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
IRY56_RS14685 3131650..3131841 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
IRY56_RS14690 3132173..3133921 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
IRY56_RS14695 3134017..3134598 + 582 WP_003414644.1 fasciclin domain-containing protein -
IRY56_RS14700 3134698..3134964 + 267 WP_031657293.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16625.74 Da        Isoelectric Point: 6.4890

>T181256 WP_011799298.1 NZ_CP064405:3130369-3130812 [Mycobacterium tuberculosis variant bovis BCG]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDTLLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T181256 NZ_CP085815:3824923-3825291 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT181256 WP_003414620.1 NZ_CP064405:3130125-3130382 [Mycobacterium tuberculosis variant bovis BCG]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT181256 NZ_CP085815:3824705-3824926 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBY8

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References